Synthesis and characterisation of acyl glycines. Their measurement in single blood spots by gas chromatography-mass spectrometry to diagnose inborn errors of metabolism

S.M.B. Carter, D.G. Watson, J.M. Midgley, R.W. Logan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Acyl glycines are normally minor metabolites of fatty acids; however, the excretion of certain acyl glycines is increased in several inborn errors of metabolism. Therefore measurement of these metabolites in body fluids can be used to diagnose these metabolic disorders. The chemical synthesis of a range of acyl glycines is described, together with that of their13C2-isotopomers for use as internal standards. An analytical method for the measurement of hexanoyl, octanoyl, 3-phenylpropionyl and suberyl glycines in urine, employing gas chromatography-mass spectrometry with negative-ion chemical ionisation was adapted to measure a larger range of acyl glycines in a single blood spot on a standard Guthrie card. Diagnoses of a case of medium-chain acyl-CoA dehydrogenase deficiency and a case of isovaleric acidaemia were confirmed using a single blood spot from each patient.
Original languageEnglish
Pages (from-to)29-35
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume677
Issue number1
DOIs
Publication statusPublished - 23 Feb 1996

Keywords

  • acylglycines
  • gas chromatography-mass spectrometry
  • chemical synthesis

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