Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endosytosis study and RNA detection

Yinan Zhang, Guoke Wei, Jun Yu, David J. S. Birch, Yu Chen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Previously we have demonstrated surface plasmon enhanced energy transfer between fluorophors and gold nanorods under two-photon excitation using fluorescence lifetime imaging microscopy (FLIM) in both solution and intracellular phases. These studies demonstrated that gold nanoparticle-dye energy transfer combinations are appealing, not only in FRET imaging, but also energy transfer-based fluorescence lifetime sensing of bio-analytes. Here, we apply this approach to study the internalization of gold nanorods (GNRs) in HeLa cells using early endosome labeling maker GFP. Observed energy transfer between GFP and GNRs indicates the involvement of endocytosis in GNR uptake. Moreover, a novel nanoprobe based on oligonucleotide functionalized gold nanorod for nucleic acid sensing via dye-GNRs energy transfer is demonstrated, potentially opening up new possibilities in cancer diagnosis and prognosis. The influence of oligonucleotide design on such nanoprobe performance was studied for the first time using time-resolved fluorescence spectroscopy, bringing new insight to the optimization of the nanoprobe.
LanguageEnglish
Pages383-394
Number of pages12
JournalFaraday Discussions
Volume178
Early online date4 Nov 2014
DOIs
Publication statusPublished - 1 Jun 2015

Fingerprint

Fluorophores
Nanorods
Gold
Energy transfer
nanorods
energy transfer
RNA
gold
Nanoprobes
oligonucleotides
Oligonucleotides
fluorescence
Coloring Agents
dyes
Fluorescence
Imaging techniques
life (durability)
prognosis
Fluorescence spectroscopy
nucleic acids

Keywords

  • gold nanorods
  • energy transfer
  • fluorescence lifetime imaging microscopy
  • RNA
  • endocytosis

Cite this

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