Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds

A.B. Jozwiak, C.M. Kielty, R.A. Black

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    Segmented polyurethanes are widely used in medical devices because of their desirable physical and chemical properties and proven biocompatibility. While polyurethane is in many respects an ideal tissue scaffold, its performance is no better than other synthetic polymers, which is due in part to its surface properties. Here, we describe a method for the functionalization of polyurethane scaffolds that involves physically incorporating another polymer (poly(ethyleneimine)) such that the surface integrity and bulk properties are retained; the primary amine groups thus incorporated into the polyurethane surface enable subsequent coupling with dextran and recombinant peptides by means of reductive amination. The efficacy of the surface functionalization of a medical grade aliphatic poly(ether)urethane is verified by surface analysis (secondary ion mass spectrometry) and fluorescence and spectrophotometric assays adapted specifically for this purpose. Further assessment of the surfaces by direct cell contact and analysis of the cellular response in terms of cell coverage and morphology before and after modification with the specific peptide sequences GRGDSPK and recombinant Fibrillin-1 fragment PF9.
    Original languageEnglish
    Pages (from-to)2240-2248
    Number of pages9
    JournalJournal of Materials Chemistry
    Volume18
    Issue number19
    Early online date26 Mar 2008
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Tissue Scaffolds
    Polyurethanes
    glycyl- arginyl-glycyl-aspartyl-seryl-prolyl-lysine
    Peptides
    Polymers
    Amination
    Dextran
    Urethane
    Surface analysis
    Secondary ion mass spectrometry
    Dextrans
    Biocompatibility
    Scaffolds
    Ether
    Chemical properties
    Amines
    Surface properties
    Ethers
    Assays
    Physical properties

    Keywords

    • biomaterial
    • microstructure
    • morphology
    • fluorescence
    • surface treatment
    • secondary ion mass spectrometry
    • surface analysis
    • peptides
    • surface properties
    • immobilization
    • polyurethane
    • functionalization

    Cite this

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    title = "Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds",
    abstract = "Segmented polyurethanes are widely used in medical devices because of their desirable physical and chemical properties and proven biocompatibility. While polyurethane is in many respects an ideal tissue scaffold, its performance is no better than other synthetic polymers, which is due in part to its surface properties. Here, we describe a method for the functionalization of polyurethane scaffolds that involves physically incorporating another polymer (poly(ethyleneimine)) such that the surface integrity and bulk properties are retained; the primary amine groups thus incorporated into the polyurethane surface enable subsequent coupling with dextran and recombinant peptides by means of reductive amination. The efficacy of the surface functionalization of a medical grade aliphatic poly(ether)urethane is verified by surface analysis (secondary ion mass spectrometry) and fluorescence and spectrophotometric assays adapted specifically for this purpose. Further assessment of the surfaces by direct cell contact and analysis of the cellular response in terms of cell coverage and morphology before and after modification with the specific peptide sequences GRGDSPK and recombinant Fibrillin-1 fragment PF9.",
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    author = "A.B. Jozwiak and C.M. Kielty and R.A. Black",
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    Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds. / Jozwiak, A.B.; Kielty, C.M.; Black, R.A.

    In: Journal of Materials Chemistry, Vol. 18, No. 19, 2008, p. 2240-2248.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds

    AU - Jozwiak, A.B.

    AU - Kielty, C.M.

    AU - Black, R.A.

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    AB - Segmented polyurethanes are widely used in medical devices because of their desirable physical and chemical properties and proven biocompatibility. While polyurethane is in many respects an ideal tissue scaffold, its performance is no better than other synthetic polymers, which is due in part to its surface properties. Here, we describe a method for the functionalization of polyurethane scaffolds that involves physically incorporating another polymer (poly(ethyleneimine)) such that the surface integrity and bulk properties are retained; the primary amine groups thus incorporated into the polyurethane surface enable subsequent coupling with dextran and recombinant peptides by means of reductive amination. The efficacy of the surface functionalization of a medical grade aliphatic poly(ether)urethane is verified by surface analysis (secondary ion mass spectrometry) and fluorescence and spectrophotometric assays adapted specifically for this purpose. Further assessment of the surfaces by direct cell contact and analysis of the cellular response in terms of cell coverage and morphology before and after modification with the specific peptide sequences GRGDSPK and recombinant Fibrillin-1 fragment PF9.

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    KW - morphology

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    KW - surface analysis

    KW - peptides

    KW - surface properties

    KW - immobilization

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    KW - functionalization

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