Surface enhanced spatially offset Raman spectroscopic (SESORS) imaging - the next dimension

Nicholas Stone, Marleen Kerssens, Gavin Rhys Lloyd, Karen Faulds, Duncan Graham, Pavel Matousek

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

SESORS - Surface enhanced spatially offset Raman spectroscopy-imaging is explored for the first time in this study. Multiplexed surface enhanced Raman scattering (SERS) signals have been recovered non-invasively from a depth of 20 mm in tissues for the first time and reconstructed to produce a false colour image. Four unique 'flavours' of SERS nanoparticles (NPs) were injected into a 20 x 50 x 50 mm porcine tissue block at the corners of a 10 mm square. A transmission Raman data cube was acquired over an 11 x 11 pixel grid made up of 2 mm steps. The signals were reconstructed using the unique peak intensities of each of the nanoparticles. A false colour image of the relative signal levels was produced, demonstrating the capability of multiplexed imaging of SERS nanoparticles using deep Raman spectroscopy. A secondary but no less significant achievement was to demonstrate that Raman signals from SERS nanoparticles can be recovered non-invasively from samples of the order of 45-50 mm thick. This is a significant step forward in the ability to detect and identify vibrational fingerprints within tissue and offers the opportunity to adapt these particles and this approach into a clinical setting for disease diagnosis.

Original languageEnglish
Pages (from-to)776-780
Number of pages5
JournalChemical Science
Volume2
Issue number4
Early online date7 Jan 2011
DOIs
Publication statusPublished - 2011

Keywords

  • silver electrode
  • raman scattering
  • sers
  • tissue
  • media
  • antibodies
  • TIC - Bionanotechnology

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