Abstract
| Language | English |
|---|---|
| Pages | 371-376 |
| Number of pages | 5 |
| Journal | Applied Spectroscopy |
| Volume | 62 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2008 |
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Keywords
- Raman spectroscopy
- surface-enhanced Raman scattering
- SERS
- folic acid
- nanoparticles
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Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum. / Stokes, R.J.; McBride, E.; Wilson, C.G.; Girkin, J.M.; Smith, W.E.; Graham, D.
In: Applied Spectroscopy, Vol. 62, No. 4, 04.2008, p. 371-376.Research output: Contribution to journal › Article
TY - JOUR
T1 - Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum
AU - Stokes, R.J.
AU - McBride, E.
AU - Wilson, C.G.
AU - Girkin, J.M.
AU - Smith, W.E.
AU - Graham, D.
N1 - http://suprimo.lib.strath.ac.uk/primo_library/libweb/action/display.do?ct=display&doc=SUVOY579586&indx=1&frbg=&dum=true&vid=SUVU01&vl(54032236UI0)=lsr02&vl(96071691UI1)=all_items&srt=rank&indx=1&dstmp=1272468249142&tab=local&ct=search&scp.scps=scope%3A(SU)&vl(freeText0)=Applied%20Spectroscopy&fn=search&mode=Basic&dscnt=0
PY - 2008/4
Y1 - 2008/4
N2 - Surface-enhanced Raman scattering (SERS) is shown to give linear and sensitive concentration-dependent detection of folic acid using silver nanoparticles created via ethylene-diaminetetraacetic acid (EDTA) reduction. Optical detection by SERS overcomes the primary limitation of photodissociation encountered during the application of other shorter wavelength ultraviolet (UV)/near-UV techniques such as fluorescence based microscopy. The SERS approach in water-based samples was demonstrated and optimized using several longer wavelengths of excitation (514.5, 632.8, and 785 nm). Excitation in the green (514.5 nm) was found to achieve the best balance between photodissociation and SERS efficiency. Linear concentration dependence was observed in the range of 0.018 to 1 lM. The importance of folic acid in a clinical setting and the potential applications of this technique in a biological environment are highlighted. We demonstrate the potential to transfer this technique to real biological samples by the detection of folic acid in human serum samples by SERS. (Abstract from : http://www.opticsinfobase.org/as/abstract.cfm?uri=as-62-4-371)
AB - Surface-enhanced Raman scattering (SERS) is shown to give linear and sensitive concentration-dependent detection of folic acid using silver nanoparticles created via ethylene-diaminetetraacetic acid (EDTA) reduction. Optical detection by SERS overcomes the primary limitation of photodissociation encountered during the application of other shorter wavelength ultraviolet (UV)/near-UV techniques such as fluorescence based microscopy. The SERS approach in water-based samples was demonstrated and optimized using several longer wavelengths of excitation (514.5, 632.8, and 785 nm). Excitation in the green (514.5 nm) was found to achieve the best balance between photodissociation and SERS efficiency. Linear concentration dependence was observed in the range of 0.018 to 1 lM. The importance of folic acid in a clinical setting and the potential applications of this technique in a biological environment are highlighted. We demonstrate the potential to transfer this technique to real biological samples by the detection of folic acid in human serum samples by SERS. (Abstract from : http://www.opticsinfobase.org/as/abstract.cfm?uri=as-62-4-371)
KW - Raman spectroscopy
KW - surface-enhanced Raman scattering
KW - SERS
KW - folic acid
KW - nanoparticles
UR - http://dx.doi.org/10.1366/000370208784046812
U2 - 10.1366/000370208784046812
DO - 10.1366/000370208784046812
M3 - Article
VL - 62
SP - 371
EP - 376
JO - Applied Spectroscopy
T2 - Applied Spectroscopy
JF - Applied Spectroscopy
SN - 0003-7028
IS - 4
ER -