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Abstract
Surface-enhanced Raman scattering (SERS) is shown to give linear and
sensitive concentration-dependent detection of folic acid using silver
nanoparticles created via ethylene-diaminetetraacetic acid (EDTA)
reduction. Optical detection by SERS overcomes the primary limitation
of photodissociation encountered during the application of other shorter
wavelength ultraviolet (UV)/near-UV techniques such as fluorescence
based microscopy. The SERS approach in water-based samples was
demonstrated and optimized using several longer wavelengths of
excitation (514.5, 632.8, and 785 nm). Excitation in the green (514.5 nm)
was found to achieve the best balance between photodissociation and
SERS efficiency. Linear concentration dependence was observed in the
range of 0.018 to 1 lM. The importance of folic acid in a clinical setting
and the potential applications of this technique in a biological
environment are highlighted. We demonstrate the potential to transfer
this technique to real biological samples by the detection of folic acid in
human serum samples by SERS.
(Abstract from : http://www.opticsinfobase.org/as/abstract.cfm?uri=as-62-4-371)
Original language | English |
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Pages (from-to) | 371-376 |
Number of pages | 5 |
Journal | Applied Spectroscopy |
Volume | 62 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2008 |
Keywords
- Raman spectroscopy
- surface-enhanced Raman scattering
- SERS
- folic acid
- nanoparticles
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Dive into the research topics of 'Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum'. Together they form a unique fingerprint.Projects
- 1 Finished
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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D. (Principal Investigator), Dawson, M. (Co-investigator), Faulds, K. (Co-investigator), Girkin, J. (Co-investigator), Graham, D. (Co-investigator), Martin, R. (Co-investigator), O'Donnell, K. (Co-investigator), Rolinski, O. (Co-investigator), Smith, W. (Co-investigator) & Wynne, K. (Co-investigator)
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research