Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum

R.J. Stokes, E. McBride, C.G. Wilson, J.M. Girkin, W.E. Smith, D. Graham

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Surface-enhanced Raman scattering (SERS) is shown to give linear and sensitive concentration-dependent detection of folic acid using silver nanoparticles created via ethylene-diaminetetraacetic acid (EDTA) reduction. Optical detection by SERS overcomes the primary limitation of photodissociation encountered during the application of other shorter wavelength ultraviolet (UV)/near-UV techniques such as fluorescence based microscopy. The SERS approach in water-based samples was demonstrated and optimized using several longer wavelengths of excitation (514.5, 632.8, and 785 nm). Excitation in the green (514.5 nm) was found to achieve the best balance between photodissociation and SERS efficiency. Linear concentration dependence was observed in the range of 0.018 to 1 lM. The importance of folic acid in a clinical setting and the potential applications of this technique in a biological environment are highlighted. We demonstrate the potential to transfer this technique to real biological samples by the detection of folic acid in human serum samples by SERS. (Abstract from : http://www.opticsinfobase.org/as/abstract.cfm?uri=as-62-4-371)
Original languageEnglish
Pages (from-to)371-376
Number of pages5
JournalApplied Spectroscopy
Volume62
Issue number4
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Raman spectroscopy
  • surface-enhanced Raman scattering
  • SERS
  • folic acid
  • nanoparticles

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