Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis

Marjorie R. Willner, Kay McMillan, Duncan Graham, Peter J. Vikesland, Michele Zagnoni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA) functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of “fast” coarse maps over an area of several mm2 followed by “slower” detailed interrogation of the identified hotspots. We demonstrate SERS at cellular resolution via a proof-of-concept assay that detects glycan expression on the surface of prostate cancer cells using WGA modified metallic nanoparticles. The data illustrates the potential of SERS optofluidic systems for high-throughput cell screening and illustrates a previously unobserved high degree of cell-to-cell variability in the size and number of glycan islands.
LanguageEnglish
JournalAnalytical Chemistry
Early online date19 Sep 2018
DOIs
Publication statusE-pub ahead of print - 19 Sep 2018

Fingerprint

Microfluidics
Raman scattering
Cells
Polysaccharides
Nanoprobes
Assays
Screening
Oils
Throughput
Nanoparticles
Water

Keywords

  • surface enhanced Raman scattering (SERS)
  • microfluidics
  • single cell analysis
  • cancer cells

Cite this

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title = "Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis",
abstract = "The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA) functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of “fast” coarse maps over an area of several mm2 followed by “slower” detailed interrogation of the identified hotspots. We demonstrate SERS at cellular resolution via a proof-of-concept assay that detects glycan expression on the surface of prostate cancer cells using WGA modified metallic nanoparticles. The data illustrates the potential of SERS optofluidic systems for high-throughput cell screening and illustrates a previously unobserved high degree of cell-to-cell variability in the size and number of glycan islands.",
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Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis. / Willner, Marjorie R.; McMillan, Kay; Graham, Duncan; Vikesland, Peter J.; Zagnoni, Michele.

In: Analytical Chemistry, 19.09.2018.

Research output: Contribution to journalArticle

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T1 - Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis

AU - Willner, Marjorie R.

AU - McMillan, Kay

AU - Graham, Duncan

AU - Vikesland, Peter J.

AU - Zagnoni, Michele

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AB - The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA) functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of “fast” coarse maps over an area of several mm2 followed by “slower” detailed interrogation of the identified hotspots. We demonstrate SERS at cellular resolution via a proof-of-concept assay that detects glycan expression on the surface of prostate cancer cells using WGA modified metallic nanoparticles. The data illustrates the potential of SERS optofluidic systems for high-throughput cell screening and illustrates a previously unobserved high degree of cell-to-cell variability in the size and number of glycan islands.

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