Supramolecular structures of enzyme clusters

Nadeem Javid, Karsten Vogtt, Sangita Roy, Andrew R. Hirst, Armin Hoell, Ian W. Hamley, Rein Vincent Ulijn, Jan Sefcik

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The structural characterization of subtilisin mesoscale clusters, which were previously shown to induce supramolecular order in biocatalytic self-assembly of Fmoc-dipeptides, was carried out by synchrotron small-angle X-ray, dynamic, and static light scattering measurements. Subtilisin molecules self-assemble to form supramolecular structures in phosphate buffer solutions. Structural arrangement of subtilisin clusters at 55degC was found to vary systematically with increasing enzyme concentration. Static light scattering measurements showed the cluster structure to be consistent with a fractal-like arrangement, with fractal dimension varying from 1.8 to 2.6 with increasing concentration for low to moderate enzyme concentrations. This was followed by a structural transition around the enzyme concentration of 0.5 mg/mL to more compact structures with significantly slower relaxation dynamics, as evidenced by dynamic light scattering measurements. These concentration-dependent supramolecular enzyme clusters provide tunable templates for biocatalytic self-assembly.
LanguageEnglish
Pages1395-1399
Number of pages5
JournalJournal of Physical Chemistry Letters
Volume2
Issue number12
Early online date18 May 2011
DOIs
Publication statusPublished - 2011

Fingerprint

Subtilisin
Enzymes
Light scattering
Self assembly
Dipeptides
Dynamic light scattering
Fractal dimension
Synchrotrons
Fractals
Buffers
Phosphates
X rays
Molecules

Keywords

  • structural characterization
  • supramolecular structures
  • enzyme clusters
  • biocatalytic self-assembly
  • TIC - Bionanotechnology

Cite this

Javid, N., Vogtt, K., Roy, S., Hirst, A. R., Hoell, A., Hamley, I. W., ... Sefcik, J. (2011). Supramolecular structures of enzyme clusters. Journal of Physical Chemistry Letters, 2(12), 1395-1399. https://doi.org/10.1021/jz200446j
Javid, Nadeem ; Vogtt, Karsten ; Roy, Sangita ; Hirst, Andrew R. ; Hoell, Armin ; Hamley, Ian W. ; Ulijn, Rein Vincent ; Sefcik, Jan. / Supramolecular structures of enzyme clusters. In: Journal of Physical Chemistry Letters. 2011 ; Vol. 2, No. 12. pp. 1395-1399.
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Javid, N, Vogtt, K, Roy, S, Hirst, AR, Hoell, A, Hamley, IW, Ulijn, RV & Sefcik, J 2011, 'Supramolecular structures of enzyme clusters' Journal of Physical Chemistry Letters, vol. 2, no. 12, pp. 1395-1399. https://doi.org/10.1021/jz200446j

Supramolecular structures of enzyme clusters. / Javid, Nadeem; Vogtt, Karsten; Roy, Sangita; Hirst, Andrew R.; Hoell, Armin; Hamley, Ian W.; Ulijn, Rein Vincent; Sefcik, Jan.

In: Journal of Physical Chemistry Letters, Vol. 2, No. 12, 2011, p. 1395-1399.

Research output: Contribution to journalArticle

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Javid N, Vogtt K, Roy S, Hirst AR, Hoell A, Hamley IW et al. Supramolecular structures of enzyme clusters. Journal of Physical Chemistry Letters. 2011;2(12):1395-1399. https://doi.org/10.1021/jz200446j