Supramolecular caging for cytosolic delivery of anionic probes

Héctor Fernández-Caro, Irene Lostalé-Seijo, Miguel Martínez-Calvo, Jesús Mosquera, José L. Mascareñas, Javier Montenegro

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)
1 Downloads (Pure)


The cytosolic delivery of hydrophilic, anionic molecular probes and therapeutics is a major challenge in chemical biology and medicine. Herein, we describe the design and synthesis of peptide-cage hybrids that allow an efficient supramolecular binding, cell membrane translocation and cytosolic delivery of a number of anionic dyes, including pyranine, carboxyfluorescein and several sulfonate-containing Alexa dyes. This supramolecular caging strategy is successful in different cell lines, and the dynamic carrier mechanism has been validated by U-tube experiments. The high efficiency of the reported approach allowed intracellular pH tracking by exploiting the ratiometric excitation of the pyranine fluorescent probe.

Original languageEnglish
Pages (from-to)8930-8938
Number of pages9
JournalChemical Science
Issue number39
Early online date20 Aug 2019
Publication statusPublished - 21 Oct 2019


  • hydrophilic small molecules
  • biopolymers
  • anionic probe


Dive into the research topics of 'Supramolecular caging for cytosolic delivery of anionic probes'. Together they form a unique fingerprint.

Cite this