Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg

N. Fontes, M.C. Almeida, S. Garcia, C. Peres, J. Partridge, P.J. Halling, S. Barreiros

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
LanguageEnglish
Pages355-358
Number of pages3
JournalBiotechnology Progress
Volume17
Issue number2
DOIs
Publication statusPublished - 3 Feb 2001

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Subtilisins
Catalysis
Enzymes
Carbonic Acid
1-Propanol
Ethane
Hexanes
Powders
Air
Acids
Water

Keywords

  • Organic-solvents
  • water activity
  • crystals
  • acetonitrile
  • hydration
  • buffers
  • ph

Cite this

Fontes, N. ; Almeida, M.C. ; Garcia, S. ; Peres, C. ; Partridge, J. ; Halling, P.J. ; Barreiros, S. / Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. In: Biotechnology Progress. 2001 ; Vol. 17, No. 2. pp. 355-358.
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Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. / Fontes, N.; Almeida, M.C.; Garcia, S.; Peres, C.; Partridge, J.; Halling, P.J.; Barreiros, S.

In: Biotechnology Progress, Vol. 17, No. 2, 03.02.2001, p. 355-358.

Research output: Contribution to journalArticle

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AU - Fontes, N.

AU - Almeida, M.C.

AU - Garcia, S.

AU - Peres, C.

AU - Partridge, J.

AU - Halling, P.J.

AU - Barreiros, S.

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AB - We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.

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KW - water activity

KW - crystals

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KW - hydration

KW - buffers

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