Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg

N. Fontes, M.C. Almeida, S. Garcia, C. Peres, J. Partridge, P.J. Halling, S. Barreiros

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We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
Original languageEnglish
Pages (from-to)355-358
Number of pages3
JournalBiotechnology Progress
Issue number2
Publication statusPublished - 3 Feb 2001


  • Organic-solvents
  • water activity
  • crystals
  • acetonitrile
  • hydration
  • buffers
  • ph

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