We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
- water activity
Fontes, N., Almeida, M. C., Garcia, S., Peres, C., Partridge, J., Halling, P. J., & Barreiros, S. (2001). Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. Biotechnology Progress, 17(2), 355-358. https://doi.org/10.1021/bp000148m