Sugar functionalised PEGA surfaces support metabolically active hepatocytes

R. F. Ambury, C. L. R. Merry, R. V. Ulijn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We demonstrate sugar functionalised hydrogel surface coatings which enable the retention of hepatocyte-specific cell function during in vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) expressed on hepatocyte cell surfaces. Polyethylene glycol acrylamide hydrogel (PEGA) was modified with a galactose containing ligand, lactobionic acid (LA) with D-glucuronic acid (GlcA) used as a non-ASGP-R binding control. FT-IR analysis of the resultant gels revealed that the sugars had been covalently incorporated into the material. Fluorescence labelling was used to quantify the degree of saccharide integration. Cell culture experiments showed that hepatocytes attached preferentially to sugar containing gel coatings, when compared to non-functionalised PEGA controls. It was observed that cells on the LA and GlcA containing surfaces were more metabolically active, compared to controls, and proliferated to a monolayer by day 7 in culture and maintained some characteristics of hepatocyte functionality, such as urea synthesis over the course of 7 days. Despite these observations, further investigations using flow cytometry and RT-PCR, proved that cell attachment was unlikely to involve an ASGP-R mediated mechanism.
LanguageEnglish
Pages2901-2908
Number of pages8
JournalJournal of Materials Chemistry
Volume21
DOIs
Publication statusPublished - 2011

Fingerprint

Acrylamide
Hydrogel
Hydrogels
Sugars
Polyethylene glycols
Asialoglycoprotein Receptor
Glucuronic Acid
Acids
Galactose
Gels
Coatings
Flow cytometry
Cell culture
Urea
Labeling
Monolayers
Adhesion
Fluorescence
Ligands
Hepatocytes

Keywords

  • binding
  • membranes
  • adhesion
  • biomaterials
  • chitosan
  • hydrogels
  • synthetic glycopolymer matrix
  • TIC - Bionanotechnology

Cite this

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title = "Sugar functionalised PEGA surfaces support metabolically active hepatocytes",
abstract = "We demonstrate sugar functionalised hydrogel surface coatings which enable the retention of hepatocyte-specific cell function during in vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) expressed on hepatocyte cell surfaces. Polyethylene glycol acrylamide hydrogel (PEGA) was modified with a galactose containing ligand, lactobionic acid (LA) with D-glucuronic acid (GlcA) used as a non-ASGP-R binding control. FT-IR analysis of the resultant gels revealed that the sugars had been covalently incorporated into the material. Fluorescence labelling was used to quantify the degree of saccharide integration. Cell culture experiments showed that hepatocytes attached preferentially to sugar containing gel coatings, when compared to non-functionalised PEGA controls. It was observed that cells on the LA and GlcA containing surfaces were more metabolically active, compared to controls, and proliferated to a monolayer by day 7 in culture and maintained some characteristics of hepatocyte functionality, such as urea synthesis over the course of 7 days. Despite these observations, further investigations using flow cytometry and RT-PCR, proved that cell attachment was unlikely to involve an ASGP-R mediated mechanism.",
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Sugar functionalised PEGA surfaces support metabolically active hepatocytes. / Ambury, R. F.; Merry, C. L. R.; Ulijn, R. V.

In: Journal of Materials Chemistry , Vol. 21, 2011, p. 2901-2908.

Research output: Contribution to journalArticle

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T1 - Sugar functionalised PEGA surfaces support metabolically active hepatocytes

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AU - Merry, C. L. R.

AU - Ulijn, R. V.

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AB - We demonstrate sugar functionalised hydrogel surface coatings which enable the retention of hepatocyte-specific cell function during in vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) expressed on hepatocyte cell surfaces. Polyethylene glycol acrylamide hydrogel (PEGA) was modified with a galactose containing ligand, lactobionic acid (LA) with D-glucuronic acid (GlcA) used as a non-ASGP-R binding control. FT-IR analysis of the resultant gels revealed that the sugars had been covalently incorporated into the material. Fluorescence labelling was used to quantify the degree of saccharide integration. Cell culture experiments showed that hepatocytes attached preferentially to sugar containing gel coatings, when compared to non-functionalised PEGA controls. It was observed that cells on the LA and GlcA containing surfaces were more metabolically active, compared to controls, and proliferated to a monolayer by day 7 in culture and maintained some characteristics of hepatocyte functionality, such as urea synthesis over the course of 7 days. Despite these observations, further investigations using flow cytometry and RT-PCR, proved that cell attachment was unlikely to involve an ASGP-R mediated mechanism.

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