Abstract
Objective
To investigate the possible role of substance P as an endothelial factor in the local regulation of vascular tone in the human ovarian vein.
Methods
We performed immunolocalization of substance P in human ovarian venous endothelium in situ and in culture, and observed responses to substance P in preconstricted ring preparations of human ovarian vein in the presence of either the prostaglandin synthesis inhibitor indomethacin or the inhibitor of nitric oxide synthesis l-nitro arginine methyl ester (l-NAME), with and without luminal rubbing.
Results
Substance P was localized in a subpopulation of ovarian vein endothelial cells. Maximal relaxation induced by substance P was not significantly affected by indomethacin (10 μmol/L), but was reduced from 58.7% (95% confidence interval [CI] 41.3–76.1) in control experiments to 24.7% (95% CI 18.3–31.1) after luminal rubbing and to 32.3% (95% CI 19.8–44.8) after exposure to l-NAME (0.1 mmol/L) (P = .001).
Conculsion
The localization of substance P in ovarian vein endothelium together with vasodilator effects mediated partially via the endothelium suggests that the peptide has a role in the local control of vascular tone in this vessel. (Obstet Gynecol 1995;85:273–8)
To investigate the possible role of substance P as an endothelial factor in the local regulation of vascular tone in the human ovarian vein.
Methods
We performed immunolocalization of substance P in human ovarian venous endothelium in situ and in culture, and observed responses to substance P in preconstricted ring preparations of human ovarian vein in the presence of either the prostaglandin synthesis inhibitor indomethacin or the inhibitor of nitric oxide synthesis l-nitro arginine methyl ester (l-NAME), with and without luminal rubbing.
Results
Substance P was localized in a subpopulation of ovarian vein endothelial cells. Maximal relaxation induced by substance P was not significantly affected by indomethacin (10 μmol/L), but was reduced from 58.7% (95% confidence interval [CI] 41.3–76.1) in control experiments to 24.7% (95% CI 18.3–31.1) after luminal rubbing and to 32.3% (95% CI 19.8–44.8) after exposure to l-NAME (0.1 mmol/L) (P = .001).
Conculsion
The localization of substance P in ovarian vein endothelium together with vasodilator effects mediated partially via the endothelium suggests that the peptide has a role in the local control of vascular tone in this vessel. (Obstet Gynecol 1995;85:273–8)
Original language | English |
---|---|
Pages (from-to) | 273-278 |
Number of pages | 6 |
Journal | Obstetrics & Gynecology |
Volume | 85 |
Issue number | 2 |
DOIs | |
Publication status | Published - 28 Feb 1995 |
Keywords
- adult
- arginine
- carboprost
- dose-response relationship
- drug
- endothelium
- vascular
- female
- humans
- immunohistochemistry
- indomethacin
- middle aged
- NG-nitroarginine methyl ester
- nitric oxide
- ovary
- substance P
- vasodilation
- veins