Abstract
The hypothesis that nicotinic agonists other than acetylcholine act by releasing endogenous acetylcholine was examined using nerve-free cultures of chick embryonic skeletal muscle. All nicotinic agonists tested (acetylcholine, carbachol, DMPP, tetramethylammonium and nicotine) depolarized the cultured skeletal muscle fibres. The depolarization was antagonised by triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium, substances that have been suggested to act by preventing agonists acting on nerve terminals. The results confirm that triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium have postjunctional blocking actions. We conclude that nicotinic agonists act directly on cholinoreceptors and do not require the presence of endogenous acetylcholine.
| Language | English |
|---|---|
| Pages | 131-134 |
| Number of pages | 4 |
| Journal | European Journal of Pharmacology |
| Volume | 28 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 1974 |
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Keywords
- acetylcholine receptors
- cultered skeletal muscle
- ganglion-blocking drugs
- hemicholinium-like drugs
- site of action
- 1,1 dimethyl 4 phenylpiperazinium
- 1,1 dimethyl 4 phenylpiperazinium iodide
- carbachol
- cholinergic receptor
- ganglion blocking agent
- hemicholinium 3
- hexamethonium
- tetramethylammonium
- drug antagonism
- in vitro study
- membrane depolarization
- muscle fiber culture
Cite this
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Studies on the pharmacology of skeletal muscle in culture : site of action of nicotinic agonists. / Harvey, Alan L.; Dryden, William F.
In: European Journal of Pharmacology, Vol. 28, No. 1, 01.01.1974, p. 131-134.Research output: Contribution to journal › Article
TY - JOUR
T1 - Studies on the pharmacology of skeletal muscle in culture
T2 - European Journal of Pharmacology
AU - Harvey, Alan L.
AU - Dryden, William F.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - The hypothesis that nicotinic agonists other than acetylcholine act by releasing endogenous acetylcholine was examined using nerve-free cultures of chick embryonic skeletal muscle. All nicotinic agonists tested (acetylcholine, carbachol, DMPP, tetramethylammonium and nicotine) depolarized the cultured skeletal muscle fibres. The depolarization was antagonised by triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium, substances that have been suggested to act by preventing agonists acting on nerve terminals. The results confirm that triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium have postjunctional blocking actions. We conclude that nicotinic agonists act directly on cholinoreceptors and do not require the presence of endogenous acetylcholine.
AB - The hypothesis that nicotinic agonists other than acetylcholine act by releasing endogenous acetylcholine was examined using nerve-free cultures of chick embryonic skeletal muscle. All nicotinic agonists tested (acetylcholine, carbachol, DMPP, tetramethylammonium and nicotine) depolarized the cultured skeletal muscle fibres. The depolarization was antagonised by triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium, substances that have been suggested to act by preventing agonists acting on nerve terminals. The results confirm that triethylcholine, hemicholinium-3, tetraethylammonium and hexamethonium have postjunctional blocking actions. We conclude that nicotinic agonists act directly on cholinoreceptors and do not require the presence of endogenous acetylcholine.
KW - acetylcholine receptors
KW - cultered skeletal muscle
KW - ganglion-blocking drugs
KW - hemicholinium-like drugs
KW - site of action
KW - 1,1 dimethyl 4 phenylpiperazinium
KW - 1,1 dimethyl 4 phenylpiperazinium iodide
KW - carbachol
KW - cholinergic receptor
KW - ganglion blocking agent
KW - hemicholinium 3
KW - hexamethonium
KW - tetramethylammonium
KW - drug antagonism
KW - in vitro study
KW - membrane depolarization
KW - muscle fiber culture
UR - http://www.scopus.com/inward/record.url?scp=0016251904&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(74)90123-X
DO - 10.1016/0014-2999(74)90123-X
M3 - Article
VL - 28
SP - 131
EP - 134
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -