Structured illumination microscopy using micro-pixellated light-emitting diodes

V. Poher, G.T. Kennedy, D.S. Elson, P.M.W. French, M.A.A. Neil, H.Z. Zhang, E. Gu, Z. Gong, C. Griffin, J.M. Girkin, M.D. Dawson

Research output: Contribution to conferencePaper

Abstract

Structured illumination is a flexible and economical method of obtaining optical sectioning in wide-field microscopy [1]. In this technique the illumination system is modified to project a single-spatial frequency grid pattern onto the sample [2, 3]. The pattern can only be resolved in the focal plane and by recording images for different transverse grid positions (or phases) an image of the in-focus parts of the object can be calculated. Light emitting diodes (LEDs) are becoming increasingly popular for lighting and illumination systems due to their low cost, small dimensions, low coherence, uniform illumination, high efficiency and long lifetime. These properties, together with recent developments in high brightness, ultraviolet operation and microstructured emitter design offer great potential for LEDs as light sources for microscopy. In this paper we demonstrate a novel structured illumination microscope using a blue micro-structured light emitting diode as the illumination source. The system is potentially very compact and has no-moving-parts.
LanguageEnglish
Publication statusUnpublished - 2006
EventPhoton 06 - Manchester, UK
Duration: 4 Sep 20067 Sep 2006

Conference

ConferencePhoton 06
CityManchester, UK
Period4/09/067/09/06

Fingerprint

light emitting diodes
illumination
microscopy
grids
illuminating
light sources
brightness
emitters
recording
microscopes
life (durability)

Keywords

  • illumination microscopy
  • microscopy
  • micro-pixellated light-emitting diode
  • light emitting diode

Cite this

Poher, V., Kennedy, G. T., Elson, D. S., French, P. M. W., Neil, M. A. A., Zhang, H. Z., ... Dawson, M. D. (2006). Structured illumination microscopy using micro-pixellated light-emitting diodes. Paper presented at Photon 06, Manchester, UK, .
Poher, V. ; Kennedy, G.T. ; Elson, D.S. ; French, P.M.W. ; Neil, M.A.A. ; Zhang, H.Z. ; Gu, E. ; Gong, Z. ; Griffin, C. ; Girkin, J.M. ; Dawson, M.D. / Structured illumination microscopy using micro-pixellated light-emitting diodes. Paper presented at Photon 06, Manchester, UK, .
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abstract = "Structured illumination is a flexible and economical method of obtaining optical sectioning in wide-field microscopy [1]. In this technique the illumination system is modified to project a single-spatial frequency grid pattern onto the sample [2, 3]. The pattern can only be resolved in the focal plane and by recording images for different transverse grid positions (or phases) an image of the in-focus parts of the object can be calculated. Light emitting diodes (LEDs) are becoming increasingly popular for lighting and illumination systems due to their low cost, small dimensions, low coherence, uniform illumination, high efficiency and long lifetime. These properties, together with recent developments in high brightness, ultraviolet operation and microstructured emitter design offer great potential for LEDs as light sources for microscopy. In this paper we demonstrate a novel structured illumination microscope using a blue micro-structured light emitting diode as the illumination source. The system is potentially very compact and has no-moving-parts.",
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Poher, V, Kennedy, GT, Elson, DS, French, PMW, Neil, MAA, Zhang, HZ, Gu, E, Gong, Z, Griffin, C, Girkin, JM & Dawson, MD 2006, 'Structured illumination microscopy using micro-pixellated light-emitting diodes' Paper presented at Photon 06, Manchester, UK, 4/09/06 - 7/09/06, .

Structured illumination microscopy using micro-pixellated light-emitting diodes. / Poher, V.; Kennedy, G.T.; Elson, D.S.; French, P.M.W.; Neil, M.A.A.; Zhang, H.Z.; Gu, E.; Gong, Z.; Griffin, C.; Girkin, J.M.; Dawson, M.D.

2006. Paper presented at Photon 06, Manchester, UK, .

Research output: Contribution to conferencePaper

TY - CONF

T1 - Structured illumination microscopy using micro-pixellated light-emitting diodes

AU - Poher, V.

AU - Kennedy, G.T.

AU - Elson, D.S.

AU - French, P.M.W.

AU - Neil, M.A.A.

AU - Zhang, H.Z.

AU - Gu, E.

AU - Gong, Z.

AU - Griffin, C.

AU - Girkin, J.M.

AU - Dawson, M.D.

PY - 2006

Y1 - 2006

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AB - Structured illumination is a flexible and economical method of obtaining optical sectioning in wide-field microscopy [1]. In this technique the illumination system is modified to project a single-spatial frequency grid pattern onto the sample [2, 3]. The pattern can only be resolved in the focal plane and by recording images for different transverse grid positions (or phases) an image of the in-focus parts of the object can be calculated. Light emitting diodes (LEDs) are becoming increasingly popular for lighting and illumination systems due to their low cost, small dimensions, low coherence, uniform illumination, high efficiency and long lifetime. These properties, together with recent developments in high brightness, ultraviolet operation and microstructured emitter design offer great potential for LEDs as light sources for microscopy. In this paper we demonstrate a novel structured illumination microscope using a blue micro-structured light emitting diode as the illumination source. The system is potentially very compact and has no-moving-parts.

KW - illumination microscopy

KW - microscopy

KW - micro-pixellated light-emitting diode

KW - light emitting diode

UR - http://photon06archive.iopconfs.org/Advanced%20imaging%20III%20Mon%2017.00.pdf

M3 - Paper

ER -

Poher V, Kennedy GT, Elson DS, French PMW, Neil MAA, Zhang HZ et al. Structured illumination microscopy using micro-pixellated light-emitting diodes. 2006. Paper presented at Photon 06, Manchester, UK, .