Structured illumination is a flexible and economical method of obtaining optical sectioning in wide-field microscopy . In this technique the illumination system is modified to project a single-spatial frequency grid pattern onto the sample [2, 3]. The pattern can only be resolved in the focal plane and by recording images for different transverse grid positions (or phases) an image of the in-focus parts of the object can be calculated. Light emitting diodes (LEDs) are becoming increasingly popular for lighting and illumination systems due to their low cost, small dimensions, low coherence, uniform illumination, high efficiency and long lifetime. These properties, together with recent developments in high brightness, ultraviolet operation and microstructured emitter design offer great potential for LEDs as light sources for microscopy. In this paper we demonstrate a novel structured illumination microscope using a blue micro-structured light emitting diode as the illumination source. The system is potentially very compact and has no-moving-parts.
|Publication status||Unpublished - 2006|
|Event||Photon 06 - Manchester, UK|
Duration: 4 Sep 2006 → 7 Sep 2006
|Period||4/09/06 → 7/09/06|
- illumination microscopy
- micro-pixellated light-emitting diode
- light emitting diode
Poher, V., Kennedy, G. T., Elson, D. S., French, P. M. W., Neil, M. A. A., Zhang, H. Z., Gu, E., Gong, Z., Griffin, C., Girkin, J. M., & Dawson, M. D. (2006). Structured illumination microscopy using micro-pixellated light-emitting diodes. Paper presented at Photon 06, Manchester, UK, .