Structural sensing using fluorescence nanotomography

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Abstract

Fluorescence nanotomography is a newly developed experimental technique enabling determination of the molecular distributions with ångstrom resolution in complex structures such as soft solids, porous materials, and biomacromolecules. In this approach to structural sensing, Fo¨rster resonance energy transfer is used as the mechanism of detection of molecular separations, and fluorescence decay measurements with nanosecond resolution are used for determination of the molecular distribution function. Unlike the traditional Fo¨rster-type approach, wherein a model fluorescence decay function is derived for an assumed donor-acceptor distribution and then fitted to the experimental decay, returning the values of the parameters of assumed distribution, fluorescence nanotomography makes no a priori assumptions regarding the distribution function. In this paper we present the theoretical background of the method and demonstrate its applicability to various molecular systems by testing the method on artificial fluorescence decay data, generated for specific molecular structures.
Original languageEnglish
Pages (from-to)10411-10418
Number of pages7
JournalJournal of Chemical Physics
Volume116
Issue number23
DOIs
Publication statusPublished - 15 Jun 2002

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Keywords

  • structural sensing
  • fluorescence nanotomography
  • nanoscience

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