Structural insights into the ene-reductase synthesis of profens

J. Waller, H. S. Toogood, V. Karuppiah, N. J. W. Rattray, D. J. Mansell, D. Leys, J. M. Gardiner, A. Fryszkowska, S. T. Ahmed, R. Bandichhor, G. P. Reddy, N. S. Scrutton

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Abstract

Reduction of double bonds of α,β-unsaturated carboxylic acids and esters by ene-reductases remains challenging and it typically requires activation by a second electron-withdrawing moiety, such as a halide or second carboxylate group. We showed that profen precursors, 2-arylpropenoic acids and their esters, were efficiently reduced by Old Yellow Enzymes (OYEs). The XenA and GYE enzymes showed activity towards acids, while a wider range of enzymes were active towards the equivalent methyl esters. Comparative co-crystal structural analysis of profen-bound OYEs highlighted key interactions important in determining substrate binding in a catalytically active conformation. The general utility of ene reductases for the synthesis of (R)-profens was established and this work will now drive future mutagenesis studies to screen for the production of pharmaceutically-active (S)-profens.
Original languageEnglish
Pages (from-to)4440-4448
Number of pages9
JournalOrganic and Biomolecular Chemistry
Volume15
Issue number20
Early online date28 Apr 2017
DOIs
Publication statusE-pub ahead of print - 28 Apr 2017

Keywords

  • carboxylic acids
  • ene-reductases
  • profen precursors
  • structural analysis

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    Waller, J., Toogood, H. S., Karuppiah, V., Rattray, N. J. W., Mansell, D. J., Leys, D., Gardiner, J. M., Fryszkowska, A., Ahmed, S. T., Bandichhor, R., Reddy, G. P., & Scrutton, N. S. (2017). Structural insights into the ene-reductase synthesis of profens. Organic and Biomolecular Chemistry, 15(20), 4440-4448. https://doi.org/10.1039/C7OB00163K