Our newly developed fluorescence resonance energy transfer (FRET) based technique, fluorescence nanotomography (FN), is used to determine the morphology and dynamics of some soft materials and bio-molecules by attaching donor (D) fluorophores and acceptors (A) to the investigated structure and using fluorescence lifetime measurements to reveal the D-A distance distribution function ρDA(r).We report the effect of the limited sizes of the donor and acceptor, effect of porous polymer, and molecular structure and phase transition in phospholipid bilayers.
- nanomolecular systems
- fluorescence nanotomography
Rolinski, O. J., Mathivanan, C., McNaught, G., & Birch, D. J. S. (2004). Structural information on nanomolecular systems revealed by FRET. Biosensors and Bioelectronics, 20(3), 424-430. https://doi.org/10.1016/j.bios.2004.02.031