Structural information on nanomolecular systems revealed by FRET

Olaf J. Rolinski, Chinnaraj Mathivanan, Gillian McNaught, David J.S. Birch

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Our newly developed fluorescence resonance energy transfer (FRET) based technique, fluorescence nanotomography (FN), is used to determine the morphology and dynamics of some soft materials and bio-molecules by attaching donor (D) fluorophores and acceptors (A) to the investigated structure and using fluorescence lifetime measurements to reveal the D-A distance distribution function ρDA(r).We report the effect of the limited sizes of the donor and acceptor, effect of porous polymer, and molecular structure and phase transition in phospholipid bilayers.
Original languageEnglish
Pages (from-to)424-430
Number of pages6
JournalBiosensors and Bioelectronics
Volume20
Issue number3
DOIs
Publication statusPublished - 25 May 2004

Keywords

  • nanomolecular systems
  • FRET
  • fluorescence nanotomography
  • nanoscience

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