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Abstract
Relative to naturally occurring Watson-Crick base pairs, the synthetic nucleotide P pairs with Z within DNA duplexes through a unique hydrogen-bond arrangement. The loss of this synthetic genetic information by PCR results in the conversion of P-Z into a G-C base pair. Here, we show structural and spectroscopic evidence that the loss of this synthetic genetic information occurs via G-Z mispairing. Remarkably, the G-Z mispair is both plastic and pH dependent; it forms a double-hydrogen-bonded “slipped” pair at pH 7.8 and a triple-hydrogen-bonded Z-G pair when the pH is above 7.8. This study highlights the need for robust structural and functional methods to elucidate the mechanisms of mutation in the development of next-generation synthetic genetic base pairs.
Original language | English |
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Pages (from-to) | 946-958 |
Number of pages | 13 |
Journal | Chem |
Volume | 1 |
Issue number | 6 |
DOIs | |
Publication status | Published - 8 Dec 2016 |
Keywords
- synthetic biology
- DNA
- base pairs
- mutation
- synthetic base pairs
- DNA structure
- x-ray crystallography
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Projects
- 1 Finished
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Is RNA splicing regulated by collisions between proteins bound to a freely-diffusing RNA chain?
Burley, G. (Principal Investigator)
1/08/14 → 30/09/17
Project: Research