Structural and mechanistic aspects of Amt/Rh proteins

Arnaud Javelle, Domenico Lupo, Xiao-Dan Li, Mike Merrick, Mohamed Chami, Pierre Ripoche, Fritz K Winkler

Research output: Contribution to journalArticle

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Abstract

Amt/Rh proteins, which mediate movement of ammonium across cell membranes, are spread throughout the three kingdoms of life. Most functional studies on various members of the family have been performed using cellular assays in heterologous expression systems, which are, however, not very well suited for detailed mechanistic studies. Although now generally considered to be ammonia conducting channels, based on a number of experimental studies and structural insights, the possibility remains that some plant Amts facilitate net ammonium ion transport. The Escherichia coli channel AmtB has become the model system of choice for analysis of the mechanism of ammonia conductance, increasingly also through molecular dynamics simulations. Further progress in a more detailed mechanistic understanding of these proteins requires a reliable in vitro assay using purified protein, allowing quantitative kinetic measurements under a variety of experimental conditions for different Amt/Rh proteins, including mutants. Here, we critically review the existing functional data in the context of the most interesting and unresolved mechanistic questions and we present our results, obtained using an in vitro assay set up with the purified E. coli channel AmtB.

LanguageEnglish
Pages472-81
Number of pages10
JournalJournal of Structural Biology
Volume158
Issue number3
DOIs
Publication statusPublished - 30 Jun 2007

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Ammonium Compounds
Ammonia
Escherichia coli
Proteins
Ion Transport
Mutant Proteins
Molecular Dynamics Simulation
Cell Membrane
In Vitro Techniques

Keywords

  • ammonia channels
  • ammonium transporters
  • Rhesus proteins
  • liposomes

Cite this

Javelle, A., Lupo, D., Li, X-D., Merrick, M., Chami, M., Ripoche, P., & Winkler, F. K. (2007). Structural and mechanistic aspects of Amt/Rh proteins. Journal of Structural Biology, 158(3), 472-81. https://doi.org/10.1016/j.jsb.2007.01.004
Javelle, Arnaud ; Lupo, Domenico ; Li, Xiao-Dan ; Merrick, Mike ; Chami, Mohamed ; Ripoche, Pierre ; Winkler, Fritz K. / Structural and mechanistic aspects of Amt/Rh proteins. In: Journal of Structural Biology. 2007 ; Vol. 158, No. 3. pp. 472-81.
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Javelle, A, Lupo, D, Li, X-D, Merrick, M, Chami, M, Ripoche, P & Winkler, FK 2007, 'Structural and mechanistic aspects of Amt/Rh proteins' Journal of Structural Biology, vol. 158, no. 3, pp. 472-81. https://doi.org/10.1016/j.jsb.2007.01.004

Structural and mechanistic aspects of Amt/Rh proteins. / Javelle, Arnaud; Lupo, Domenico; Li, Xiao-Dan; Merrick, Mike; Chami, Mohamed; Ripoche, Pierre; Winkler, Fritz K.

In: Journal of Structural Biology, Vol. 158, No. 3, 30.06.2007, p. 472-81.

Research output: Contribution to journalArticle

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