Stroke penumbra defined by an MRI-based oxygen challenge technique: 2. Validation based on the consequences of reperfusion

Craig A Robertson, Christopher McCabe, Lindsay Gallagher, Maria del Rosario Lopez-Gonzalez, William M Holmes, Barrie Condon, Keith W Muir, Celestine Santosh, I Mhairi Macrae

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) with oxygen challenge (T(2)(*) OC) uses oxygen as a metabolic biotracer to define penumbral tissue based on CMRO(2) and oxygen extraction fraction. Penumbra displays a greater T(2)(*) signal change during OC than surrounding tissue. Since timely restoration of cerebral blood flow (CBF) should salvage penumbra, T(2)(*) OC was tested by examining the consequences of reperfusion on T(2)(*) OC-defined penumbra. Transient ischemia (109 ± 20 minutes) was induced in male Sprague-Dawley rats (n=8). Penumbra was identified on T(2)(*)-weighted MRI during OC. Ischemia and ischemic injury were identified on CBF and apparent diffusion coefficient maps, respectively. Reperfusion was induced and scans repeated. T(2) for final infarct and T(2)(*) OC were run on day 7. T(2)(*) signal increase to OC was 3.4% in contralateral cortex and caudate nucleus and was unaffected by reperfusion. In OC-defined penumbra, T(2)(*) signal increased by 8.4% ± 4.1% during ischemia and returned to 3.25% ± 0.8% following reperfusion. Ischemic core T(2)(*) signal increase was 0.39% ± 0.47% during ischemia and 0.84% ± 1.8% on reperfusion. Penumbral CBF increased from 41.94 ± 13 to 116.5 ± 25 mL per 100 g per minute on reperfusion. On day 7, OC-defined penumbra gave a normal OC response and was located outside the infarct. T(2)(*) OC-defined penumbra recovered when CBF was restored, providing further validation of the utility of T(2)(*) OC for acute stroke management.

LanguageEnglish
Pages1788-1798
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume31
Issue number8
Early online date11 May 2011
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Cerebrovascular Circulation
Magnetic resonance imaging
Reperfusion
Blood
Stroke
Magnetic Resonance Imaging
Oxygen
Ischemia
Tissue
Salvaging
Restoration
Rats
Caudate Nucleus
Sprague Dawley Rats
Wounds and Injuries

Keywords

  • animals
  • brain ischemia
  • cerebrovascular circulation
  • iIschemic attack, transient
  • Magnetic resonance imaging
  • male
  • oxygen
  • rats
  • stroke

Cite this

Robertson, Craig A ; McCabe, Christopher ; Gallagher, Lindsay ; Lopez-Gonzalez, Maria del Rosario ; Holmes, William M ; Condon, Barrie ; Muir, Keith W ; Santosh, Celestine ; Macrae, I Mhairi. / Stroke penumbra defined by an MRI-based oxygen challenge technique : 2. Validation based on the consequences of reperfusion. In: Journal of Cerebral Blood Flow and Metabolism. 2011 ; Vol. 31, No. 8. pp. 1788-1798.
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title = "Stroke penumbra defined by an MRI-based oxygen challenge technique: 2. Validation based on the consequences of reperfusion",
abstract = "Magnetic resonance imaging (MRI) with oxygen challenge (T(2)(*) OC) uses oxygen as a metabolic biotracer to define penumbral tissue based on CMRO(2) and oxygen extraction fraction. Penumbra displays a greater T(2)(*) signal change during OC than surrounding tissue. Since timely restoration of cerebral blood flow (CBF) should salvage penumbra, T(2)(*) OC was tested by examining the consequences of reperfusion on T(2)(*) OC-defined penumbra. Transient ischemia (109 ± 20 minutes) was induced in male Sprague-Dawley rats (n=8). Penumbra was identified on T(2)(*)-weighted MRI during OC. Ischemia and ischemic injury were identified on CBF and apparent diffusion coefficient maps, respectively. Reperfusion was induced and scans repeated. T(2) for final infarct and T(2)(*) OC were run on day 7. T(2)(*) signal increase to OC was 3.4{\%} in contralateral cortex and caudate nucleus and was unaffected by reperfusion. In OC-defined penumbra, T(2)(*) signal increased by 8.4{\%} ± 4.1{\%} during ischemia and returned to 3.25{\%} ± 0.8{\%} following reperfusion. Ischemic core T(2)(*) signal increase was 0.39{\%} ± 0.47{\%} during ischemia and 0.84{\%} ± 1.8{\%} on reperfusion. Penumbral CBF increased from 41.94 ± 13 to 116.5 ± 25 mL per 100 g per minute on reperfusion. On day 7, OC-defined penumbra gave a normal OC response and was located outside the infarct. T(2)(*) OC-defined penumbra recovered when CBF was restored, providing further validation of the utility of T(2)(*) OC for acute stroke management.",
keywords = "animals, brain ischemia, cerebrovascular circulation, iIschemic attack, transient, Magnetic resonance imaging, male, oxygen, rats, stroke",
author = "Robertson, {Craig A} and Christopher McCabe and Lindsay Gallagher and Lopez-Gonzalez, {Maria del Rosario} and Holmes, {William M} and Barrie Condon and Muir, {Keith W} and Celestine Santosh and Macrae, {I Mhairi}",
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Robertson, CA, McCabe, C, Gallagher, L, Lopez-Gonzalez, MDR, Holmes, WM, Condon, B, Muir, KW, Santosh, C & Macrae, IM 2011, 'Stroke penumbra defined by an MRI-based oxygen challenge technique: 2. Validation based on the consequences of reperfusion' Journal of Cerebral Blood Flow and Metabolism, vol. 31, no. 8, pp. 1788-1798. https://doi.org/10.1038/jcbfm.2011.67

Stroke penumbra defined by an MRI-based oxygen challenge technique : 2. Validation based on the consequences of reperfusion. / Robertson, Craig A; McCabe, Christopher; Gallagher, Lindsay; Lopez-Gonzalez, Maria del Rosario; Holmes, William M; Condon, Barrie; Muir, Keith W; Santosh, Celestine; Macrae, I Mhairi.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 31, No. 8, 08.2011, p. 1788-1798.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stroke penumbra defined by an MRI-based oxygen challenge technique

T2 - Journal of Cerebral Blood Flow and Metabolism

AU - Robertson, Craig A

AU - McCabe, Christopher

AU - Gallagher, Lindsay

AU - Lopez-Gonzalez, Maria del Rosario

AU - Holmes, William M

AU - Condon, Barrie

AU - Muir, Keith W

AU - Santosh, Celestine

AU - Macrae, I Mhairi

PY - 2011/8

Y1 - 2011/8

N2 - Magnetic resonance imaging (MRI) with oxygen challenge (T(2)(*) OC) uses oxygen as a metabolic biotracer to define penumbral tissue based on CMRO(2) and oxygen extraction fraction. Penumbra displays a greater T(2)(*) signal change during OC than surrounding tissue. Since timely restoration of cerebral blood flow (CBF) should salvage penumbra, T(2)(*) OC was tested by examining the consequences of reperfusion on T(2)(*) OC-defined penumbra. Transient ischemia (109 ± 20 minutes) was induced in male Sprague-Dawley rats (n=8). Penumbra was identified on T(2)(*)-weighted MRI during OC. Ischemia and ischemic injury were identified on CBF and apparent diffusion coefficient maps, respectively. Reperfusion was induced and scans repeated. T(2) for final infarct and T(2)(*) OC were run on day 7. T(2)(*) signal increase to OC was 3.4% in contralateral cortex and caudate nucleus and was unaffected by reperfusion. In OC-defined penumbra, T(2)(*) signal increased by 8.4% ± 4.1% during ischemia and returned to 3.25% ± 0.8% following reperfusion. Ischemic core T(2)(*) signal increase was 0.39% ± 0.47% during ischemia and 0.84% ± 1.8% on reperfusion. Penumbral CBF increased from 41.94 ± 13 to 116.5 ± 25 mL per 100 g per minute on reperfusion. On day 7, OC-defined penumbra gave a normal OC response and was located outside the infarct. T(2)(*) OC-defined penumbra recovered when CBF was restored, providing further validation of the utility of T(2)(*) OC for acute stroke management.

AB - Magnetic resonance imaging (MRI) with oxygen challenge (T(2)(*) OC) uses oxygen as a metabolic biotracer to define penumbral tissue based on CMRO(2) and oxygen extraction fraction. Penumbra displays a greater T(2)(*) signal change during OC than surrounding tissue. Since timely restoration of cerebral blood flow (CBF) should salvage penumbra, T(2)(*) OC was tested by examining the consequences of reperfusion on T(2)(*) OC-defined penumbra. Transient ischemia (109 ± 20 minutes) was induced in male Sprague-Dawley rats (n=8). Penumbra was identified on T(2)(*)-weighted MRI during OC. Ischemia and ischemic injury were identified on CBF and apparent diffusion coefficient maps, respectively. Reperfusion was induced and scans repeated. T(2) for final infarct and T(2)(*) OC were run on day 7. T(2)(*) signal increase to OC was 3.4% in contralateral cortex and caudate nucleus and was unaffected by reperfusion. In OC-defined penumbra, T(2)(*) signal increased by 8.4% ± 4.1% during ischemia and returned to 3.25% ± 0.8% following reperfusion. Ischemic core T(2)(*) signal increase was 0.39% ± 0.47% during ischemia and 0.84% ± 1.8% on reperfusion. Penumbral CBF increased from 41.94 ± 13 to 116.5 ± 25 mL per 100 g per minute on reperfusion. On day 7, OC-defined penumbra gave a normal OC response and was located outside the infarct. T(2)(*) OC-defined penumbra recovered when CBF was restored, providing further validation of the utility of T(2)(*) OC for acute stroke management.

KW - animals

KW - brain ischemia

KW - cerebrovascular circulation

KW - iIschemic attack, transient

KW - Magnetic resonance imaging

KW - male

KW - oxygen

KW - rats

KW - stroke

U2 - 10.1038/jcbfm.2011.67

DO - 10.1038/jcbfm.2011.67

M3 - Article

VL - 31

SP - 1788

EP - 1798

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 8

ER -