Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor

Penelope M. Tsimbouri, Peter G. Childs, Gabriel D. Pemberton, Jingli Yang, Vineetha Jayawarna, Wich Orapiriyakul, Karl Burgess, Cristina González-García, Gavin Blackburn, Dilip Thomas, Catalina Vallejo-Giraldo, Manus J. P Biggs, Adam S. G. Curtis, Manuel Salmerón-Sánchez, Stuart Reid, Matthew J. Dalby

Research output: Contribution to journalArticle

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Abstract

Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.
Original languageEnglish
Pages (from-to)758-770
Number of pages13
JournalNature Biomedical Engineering
Volume1
Issue number9
DOIs
Publication statusPublished - 12 Sep 2017

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Bioreactors
Stem cells
Mesenchymal Stromal Cells
Osteogenesis
Grafts
Bone
Tissue
Transplants
Gels
Stiffness
Bone and Bones
Preclinical Drug Evaluations
Vibration
Collagen
Scaffolds
Rigidity
Screening
Morbidity
Pain
Pharmaceutical Preparations

Keywords

  • mesenchymal stem cells
  • tissue engineering
  • nanobiotechnology

Cite this

Tsimbouri, P. M., Childs, P. G., Pemberton, G. D., Yang, J., Jayawarna, V., Orapiriyakul, W., ... Dalby, M. J. (2017). Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nature Biomedical Engineering, 1(9), 758-770. https://doi.org/10.1038/s41551-017-0127-4
Tsimbouri, Penelope M. ; Childs, Peter G. ; Pemberton, Gabriel D. ; Yang, Jingli ; Jayawarna, Vineetha ; Orapiriyakul, Wich ; Burgess, Karl ; González-García, Cristina ; Blackburn, Gavin ; Thomas, Dilip ; Vallejo-Giraldo, Catalina ; Biggs, Manus J. P ; Curtis, Adam S. G. ; Salmerón-Sánchez, Manuel ; Reid, Stuart ; Dalby, Matthew J. / Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. In: Nature Biomedical Engineering. 2017 ; Vol. 1, No. 9. pp. 758-770.
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abstract = "Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.",
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Tsimbouri, PM, Childs, PG, Pemberton, GD, Yang, J, Jayawarna, V, Orapiriyakul, W, Burgess, K, González-García, C, Blackburn, G, Thomas, D, Vallejo-Giraldo, C, Biggs, MJP, Curtis, ASG, Salmerón-Sánchez, M, Reid, S & Dalby, MJ 2017, 'Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor', Nature Biomedical Engineering, vol. 1, no. 9, pp. 758-770. https://doi.org/10.1038/s41551-017-0127-4

Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. / Tsimbouri, Penelope M.; Childs, Peter G.; Pemberton, Gabriel D.; Yang, Jingli; Jayawarna, Vineetha; Orapiriyakul, Wich; Burgess, Karl; González-García, Cristina; Blackburn, Gavin; Thomas, Dilip; Vallejo-Giraldo, Catalina; Biggs, Manus J. P; Curtis, Adam S. G.; Salmerón-Sánchez, Manuel; Reid, Stuart; Dalby, Matthew J.

In: Nature Biomedical Engineering, Vol. 1, No. 9, 12.09.2017, p. 758-770.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor

AU - Tsimbouri, Penelope M.

AU - Childs, Peter G.

AU - Pemberton, Gabriel D.

AU - Yang, Jingli

AU - Jayawarna, Vineetha

AU - Orapiriyakul, Wich

AU - Burgess, Karl

AU - González-García, Cristina

AU - Blackburn, Gavin

AU - Thomas, Dilip

AU - Vallejo-Giraldo, Catalina

AU - Biggs, Manus J. P

AU - Curtis, Adam S. G.

AU - Salmerón-Sánchez, Manuel

AU - Reid, Stuart

AU - Dalby, Matthew J.

N1 - The deposited file includes supplementary information relevant to the article proper.

PY - 2017/9/12

Y1 - 2017/9/12

N2 - Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.

AB - Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.

KW - mesenchymal stem cells

KW - tissue engineering

KW - nanobiotechnology

U2 - 10.1038/s41551-017-0127-4

DO - 10.1038/s41551-017-0127-4

M3 - Article

VL - 1

SP - 758

EP - 770

JO - Nature Biomedical Engineering

JF - Nature Biomedical Engineering

SN - 2157-846X

IS - 9

ER -

Tsimbouri PM, Childs PG, Pemberton GD, Yang J, Jayawarna V, Orapiriyakul W et al. Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nature Biomedical Engineering. 2017 Sep 12;1(9):758-770. https://doi.org/10.1038/s41551-017-0127-4