Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

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Abstract

Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.
Original languageEnglish
Number of pages1
Publication statusPublished - 5 Jun 2018
Event18th European Light Microscopy Initiative Meeting 2018 - Dublin, Ireland
Duration: 5 Jun 20188 Jun 2018
Conference number: 18th
https://www.elmi2018.eu/

Conference

Conference18th European Light Microscopy Initiative Meeting 2018
Abbreviated titleELMI 2018
CountryIreland
CityDublin
Period5/06/188/06/18
Internet address

Keywords

  • widefield fluorescence microscopy
  • standing wave microscopy
  • red blood cells

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    Tinning, P. W., Scrimgeour, R., & McConnell, G. (2018). Standing wave microscopy of red blood cell membrane morphology with high temporal resolution. Poster session presented at 18th European Light Microscopy Initiative Meeting 2018, Dublin, Ireland.