Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Research output: Contribution to conferencePoster

Abstract

Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.
LanguageEnglish
Number of pages1
Publication statusPublished - 5 Jun 2018
EventELMI 2018 - Dublin, Ireland
Duration: 5 Jun 20188 Jun 2018

Conference

ConferenceELMI 2018
CountryIreland
CityDublin
Period5/06/188/06/18

Fingerprint

Biological Science Disciplines
erythrocytes
Cell membranes
Lighting
temporal resolution
Fluorescence Microscopy
standing waves
Microscopy
Microscopic examination
Blood
Erythrocytes
Fluorescence
Cell Membrane
microscopy
Light
Fluorophores
Fluorescence microscopy
high resolution
antinodes
fluorescence

Keywords

  • widefield fluorescence microscopy
  • standing wave microscopy
  • red blood cells

Cite this

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title = "Standing wave microscopy of red blood cell membrane morphology with high temporal resolution",
abstract = "Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.",
keywords = "widefield fluorescence microscopy, standing wave microscopy, red blood cells",
author = "Tinning, {Peter W.} and Ross Scrimgeour and Gail McConnell",
year = "2018",
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language = "English",
note = "ELMI 2018 ; Conference date: 05-06-2018 Through 08-06-2018",

}

Standing wave microscopy of red blood cell membrane morphology with high temporal resolution. / Tinning, Peter W.; Scrimgeour, Ross; McConnell, Gail.

2018. Poster session presented at ELMI 2018, Dublin, Ireland.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

AU - Tinning, Peter W.

AU - Scrimgeour, Ross

AU - McConnell, Gail

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.

AB - Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.

KW - widefield fluorescence microscopy

KW - standing wave microscopy

KW - red blood cells

M3 - Poster

ER -