Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Peter W. Tinning; Ross Scrimgeour; Gail McConnell

Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Peter W. Tinning, Ross Scrimgeour, Gail McConnell

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Abstract

Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.

Original language	English
Number of pages	1
Publication status	Published - 5 Jun 2018
Event	18th European Light Microscopy Initiative Meeting 2018 - Dublin, Ireland Duration: 5 Jun 2018 → 8 Jun 2018 Conference number: 18th https://www.elmi2018.eu/

Conference

Conference	18th European Light Microscopy Initiative Meeting 2018
Abbreviated title	ELMI 2018
Country/Territory	Ireland
City	Dublin
Period	5/06/18 → 8/06/18
Internet address	https://www.elmi2018.eu/

Keywords

widefield fluorescence microscopy
standing wave microscopy
red blood cells

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Tinning-etal-EMLI-2018-Standing-wave-microscopy-of-red-blood-cell-membrane-morphologyAccepted author manuscript, 995 KBLicence: CC BY-NC-SA 4.0

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title = "Standing wave microscopy of red blood cell membrane morphology with high temporal resolution",

abstract = "Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.",

keywords = "widefield fluorescence microscopy, standing wave microscopy, red blood cells",

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T1 - Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

AU - Tinning, Peter W.

AU - Scrimgeour, Ross

AU - McConnell, Gail

N1 - Conference code: 18th

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.

AB - Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.

KW - widefield fluorescence microscopy

KW - standing wave microscopy

KW - red blood cells

M3 - Poster

T2 - 18th European Light Microscopy Initiative Meeting 2018

Y2 - 5 June 2018 through 8 June 2018

ER -

Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Abstract

Conference

Keywords

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