Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor, Sumeet Mahajan, William B. Amos, Gail McConnell

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9330
DOIs
Publication statusPublished - 9 Mar 2015
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII - San Francisco, United States
Duration: 9 Feb 201512 Feb 2015

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
CountryUnited States
CitySan Francisco
Period9/02/1512/02/15

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Keywords

  • cell membrane
  • fluorescence microscopy
  • laser-scanning
  • standing-wave excitation

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