Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor; Sumeet Mahajan; William B. Amos; Gail McConnell

doi:10.1117/12.2075644

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor, Sumeet Mahajan, William B. Amos, Gail McConnell

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9330
DOIs	https://doi.org/10.1117/12.2075644
Publication status	Published - 9 Mar 2015
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII - San Francisco, United States Duration: 9 Feb 2015 → 12 Feb 2015

Conference

Conference	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
Country/Territory	United States
City	San Francisco
Period	9/02/15 → 12/02/15

Keywords

cell membrane
fluorescence microscopy
laser-scanning
standing-wave excitation

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10.1117/12.2075644

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title = "Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane",

abstract = "We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moir{\'e} pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.",

keywords = "cell membrane, fluorescence microscopy, laser-scanning, standing-wave excitation",

author = "Rumelo Amor and Sumeet Mahajan and Amos, {William B.} and Gail McConnell",

year = "2015",

month = mar,

day = "9",

doi = "10.1117/12.2075644",

language = "English",

isbn = "9781628414202",

volume = "9330",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

note = "Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII ; Conference date: 09-02-2015 Through 12-02-2015",

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Amor, R, Mahajan, S, Amos, WB & McConnell, G 2015, Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9330, 933010, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, San Francisco, United States, 9/02/15. https://doi.org/10.1117/12.2075644

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. / Amor, Rumelo; Mahajan, Sumeet; Amos, William B. et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9330 2015. 933010.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

TY - GEN

T1 - Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

AU - Amor, Rumelo

AU - Mahajan, Sumeet

AU - Amos, William B.

AU - McConnell, Gail

PY - 2015/3/9

Y1 - 2015/3/9

N2 - We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

AB - We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

KW - cell membrane

KW - fluorescence microscopy

KW - laser-scanning

KW - standing-wave excitation

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U2 - 10.1117/12.2075644

DO - 10.1117/12.2075644

M3 - Conference contribution book

AN - SCOPUS:84931846948

SN - 9781628414202

VL - 9330

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII

Y2 - 9 February 2015 through 12 February 2015

ER -

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

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Keywords

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