Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor, Sumeet Mahajan, William B. Amos, Gail McConnell

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

LanguageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9330
DOIs
Publication statusPublished - 9 Mar 2015
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII - San Francisco, United States
Duration: 9 Feb 201512 Feb 2015

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
CountryUnited States
CitySan Francisco
Period9/02/1512/02/15

Fingerprint

wave excitation
erythrocytes
Cell membranes
standing waves
Moire Topography
Lasers
Microscopes
Blood
Erythrocytes
Fluorescence
microscopes
Cell Membrane
Scanning
fluorescence
scanning
lasers
Mirrors
mirrors
Membranes
excitation

Keywords

  • cell membrane
  • fluorescence microscopy
  • laser-scanning
  • standing-wave excitation

Cite this

Amor, Rumelo ; Mahajan, Sumeet ; Amos, William B. ; McConnell, Gail. / Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9330 2015.
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Amor, R, Mahajan, S, Amos, WB & McConnell, G 2015, Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9330, 933010, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, San Francisco, United States, 9/02/15. https://doi.org/10.1117/12.2075644

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. / Amor, Rumelo; Mahajan, Sumeet; Amos, William B.; McConnell, Gail.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9330 2015. 933010.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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