Stability of protein-coated microcrystals in organic solvents

M. Kreiner, J.F.A. Fernandes, N. O'Farrell, P.J. Halling, M.C. Parker

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previously we reported a new high activity biocatalyst for use in organic media, termed protein-coated microcrystals (PCMC) [M. Kreiner, B.D. Moore, M.C. Parker, Chem. Commun. 12 (2001) 1906]. These novel biocomposites consist of water-soluble micron-sized crystalline particles coated with the given biocatalyst(s). Here we have looked at the stability of PCMC and their catalytic behaviour as a function of temperature in different organic media. PCMC show very good long-term stability at room temperature, when stored as suspensions in 1-propanol/I wt.% H2O. Candida antarctica lipase B and subtilisin Carlsberg (SC) in PCMC form retained nearly 90% of their initial activity after 1 year at room temperature (RT). The effects of temperature on the catalytic activity of SC-PCMC are solvent-dependant. In 1-propanol/1 wt.% H2O, the initial rate increased when the temperature was elevated from 25 to 60 degrees C, whereas in acetonitrile/1 wt.% H2O, SC-PCMC lost activity. The operational stability of PCMC is also solvent-dependant. In 1-propanol/I wt.% H2O, SC-PCMC lost only 16% of the initial activity after five batch cycles. Rather poor stability was found for SC-PCMC in THF/1% (v/v) H2O and acetonitrile/1% (v/v) H2O, with a rapid loss of activity within 4h in a continuous flow reactor. However, during the next 4 days only a slow further deactivation was observed.
Original languageEnglish
Pages (from-to)65-72
Number of pages7
JournalJournal of Molecular Catalysis B: Enzymatic
Volume33
Issue number3-6
DOIs
Publication statusPublished - 1 Jun 2005

Keywords

  • protein-coated microcrystals(PCMC)
  • crystalline particles
  • organic media
  • antarctica lipase B
  • Stability
  • Organic solvent
  • Subtilisin
  • Lipase

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