Stability-indicating micellar enhanced spectro-fluorometric determination of Daclatasvir in its tablet and spiked human plasma

Maha A. Sultan, Maha M. Abou El-Alamin, Alastair W. Wark, Marwa M. Azab

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Abstract

A fast, simple and sensitive micellar enhanced spectrofluorimetric method is performed for the determination of Daclatasvir dihydrochloride (DAC) in its pharmaceutical dosage form and in spiked human plasma. The fluorescence intensity (FI) was measured at 367 nm after excitation at 300 nm. In aqueous solution, the FI of DAC was greatly enhanced by >110% in the presence of sodium dodecyl sulphate (SDS). The detection method was linear over the range of 12.93 to 161.60 ng/mL, with a limit of detection of 1.75 ng/mL. The proposed method was successfully applied to the determination of DAC in its pharmaceutical dosage form and the mean % recovery of DAC in spiked human plasma was 95.42 ± 2.52. The developed methodology was also extended to stress studies of DAC after exposure to different forced degradation conditions including acidic, alkaline, photolytic, thermal and oxidative environments.
Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume211
Early online date17 Nov 2018
DOIs
Publication statusPublished - 15 Mar 2019

Keywords

  • Daclatasvir dihydrochloride (DAC) detection
  • spectrofluorimetry
  • spiked human plasma

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