Sphingosine kinase 1 is an intracellular effector of phosphatidic acid

C. Delon, M. Manifava, E. Wood, D. Thompson, S. Krugmann, S. Pyne, N.T. Ktistakis

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Abstract

Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. The SK1-PA interaction maps to the C terminus of SK1 and is independent of catalytic activity or of the diacylglycerol kinase-like domain of the enzyme. Thus SK1 constitutes a novel, physiologically relevant PA effector.
Original languageEnglish
Pages (from-to)44763-44774
Number of pages12
JournalJournal of Biological Chemistry
Volume279
DOIs
Publication statusPublished - 2004

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Keywords

  • sphingosine kinase 1
  • intracellular effector
  • phosphatidic acid

Cite this

Delon, C., Manifava, M., Wood, E., Thompson, D., Krugmann, S., Pyne, S., & Ktistakis, N. T. (2004). Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. Journal of Biological Chemistry, 279, 44763-44774. https://doi.org/10.1074/jbc.M405771200