Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1 % w/v suspension in an aqueous 10 mm phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.
|Number of pages||4|
|Publication status||Published - May 2002|
- capillary electrokinetic chromatography
- molecular recognition
- molecularly imprinted polymers
de Boer, T., Mol, R., de Zeeuw, R. A., de Jong, G. J., Sherrington, D. C., Cormack, P. A. G., & Ensing, K. (2002). Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations. Electrophoresis, 23(9), 1296-1300. https://doi.org/10.1002/1522-2683(200205)23:9<1296::AID-ELPS1296>3.0.CO;2-2