Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations

T. de Boer, R. Mol, R.A. de Zeeuw, G.J. de Jong, D.C. Sherrington, P.A.G. Cormack, K. Ensing

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1 % w/v suspension in an aqueous 10 mm phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.
LanguageEnglish
Pages1296-1300
Number of pages4
JournalElectrophoresis
Volume23
Issue number9
DOIs
Publication statusPublished - May 2002

Fingerprint

Ephedrine
Capillary electrophoresis
Molecular recognition
Capillary Electrophoresis
Polymers
Microspheres
Polymerization
Suspensions
Buffers
Phosphates
acetonitrile
methacrylic acid

Keywords

  • capillary electrokinetic chromatography
  • molecular recognition
  • molecularly imprinted polymers

Cite this

de Boer, T. ; Mol, R. ; de Zeeuw, R.A. ; de Jong, G.J. ; Sherrington, D.C. ; Cormack, P.A.G. ; Ensing, K. / Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations. In: Electrophoresis. 2002 ; Vol. 23, No. 9. pp. 1296-1300.
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Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations. / de Boer, T.; Mol, R.; de Zeeuw, R.A.; de Jong, G.J.; Sherrington, D.C.; Cormack, P.A.G.; Ensing, K.

In: Electrophoresis, Vol. 23, No. 9, 05.2002, p. 1296-1300.

Research output: Contribution to journalArticle

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AU - de Boer, T.

AU - Mol, R.

AU - de Zeeuw, R.A.

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AU - Sherrington, D.C.

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KW - molecularly imprinted polymers

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