TY - GEN
T1 - Spermatozoa quality assessment
T2 - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
AU - De Angelis, Annalisa
AU - Ferrara, Maria A.
AU - Di Caprio, Giuseppe
AU - Managò, Stefano
AU - Sirleto, Luigi
AU - Coppola, Giuseppe
AU - De Luca, Anna Chiara
N1 - Publisher Copyright: © 2015 SPIE.
Annalisa De Angelis, Maria Antonietta Ferrara, Giuseppe Di Caprio, Stefano Managò, Luigi Sirleto, Giuseppe Coppola, Anna Chiara De Luca, "Spermatozoa quality assessment: a combined holographic and Raman microscopy approach," Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 952916 (22 June 2015); https://doi.org/10.1117/12.2186393
PY - 2015/6/22
Y1 - 2015/6/22
N2 - Semen analysis is widely used as diagnostic tool for assessing male fertility, controlling and managing the animal reproduction. The most important parameters measured in a semen analysis are the morphology and biochemical alterations. For obtaining such information, non-invasive, label-free and non-destructive techniques have to be used. Digital Holography (DH) combined with Raman Spectroscopy (RS) could represent the perfect candidate for a rapid, non-destructive and high-sensitive morphological and biochemical sperm cell analysis. In this study, DH-RS combined approach is used for a complete analysis of single bovine spermatozoa. High-resolution images of bovine sperm have been obtained by DH microscopy from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Such anomalies have been also confirmed by Raman imaging analysis, suggesting the protein vibrations as associated Raman marker of the defect.
AB - Semen analysis is widely used as diagnostic tool for assessing male fertility, controlling and managing the animal reproduction. The most important parameters measured in a semen analysis are the morphology and biochemical alterations. For obtaining such information, non-invasive, label-free and non-destructive techniques have to be used. Digital Holography (DH) combined with Raman Spectroscopy (RS) could represent the perfect candidate for a rapid, non-destructive and high-sensitive morphological and biochemical sperm cell analysis. In this study, DH-RS combined approach is used for a complete analysis of single bovine spermatozoa. High-resolution images of bovine sperm have been obtained by DH microscopy from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Such anomalies have been also confirmed by Raman imaging analysis, suggesting the protein vibrations as associated Raman marker of the defect.
KW - biophotonics
KW - digital holography microscopy
KW - Raman imaging
KW - Raman spectroscopy
KW - spermatozoa
KW - computer generated holograph
KW - nondestructive examination
KW - digital holography microscopies
UR - http://www.scopus.com/inward/record.url?scp=84938884808&partnerID=8YFLogxK
U2 - 10.1117/12.2186393
DO - 10.1117/12.2186393
M3 - Conference contribution book
AN - SCOPUS:84938884808
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
A2 - Grilli, Simonetta
A2 - Ferraro, Pietro
A2 - Ritsch-Marte, Monika
A2 - Stifter, David
Y2 - 22 June 2015 through 24 June 2015
ER -