Spectroscopic investigations of surface deposited biological warfare simulants

Stephen J. Barrington, Hilary Bird, Daniel Hurst, Alastair J S McIntosh, Phillippa Spencer, Suzanne H. Pelfrey, Matthew J. Baker

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This paper reports a proof-of-principle study aimed at discriminating biological warfare (BW) simulants from common environmental bacteria in order to differentiate pathogenic endospores in situ, to aid any required response for hazard management. We used FTIR spectroscopy combined with multivariate analysis; FTIR is a versatile technique for the non-destructive analysis of a range of materials. We also report an evaluation of multiple pre-processing techniques and subsequent differences in cross-validation accuracy of two pattern recognition models (Support Vector Machines (SVM) and Principal Component-Linear Discriminant Analysis (PC-LDA)) for two classifications: a two class classification (Gram + ve spores vs. Gram -ve vegetative cells) and a six class classification (bacterial classification). Six bacterial strains Bacillus atrophaeus, Bacillus thuringiensis var. kurstaki, Bacillus thuringiensis, Escherichia coli, Pantaeoa agglomerans and Pseudomonas fluorescens were analysed. 

Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationChemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Place of PublicationBellingham, Washington USA
Number of pages8
Volume8358
DOIs
Publication statusPublished - 3 Dec 2012
EventChemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII - Baltimore, MD, United Kingdom
Duration: 24 Apr 201227 Apr 2012

Conference

ConferenceChemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
CountryUnited Kingdom
CityBaltimore, MD
Period24/04/1227/04/12

Keywords

  • bacteria
  • biological warfare
  • infrared spectroscopy
  • pre-processing
  • Surface deposited. Bacillus

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