Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Mhairi M. Harper, Barry Robertson, Alastair Ricketts, Karen Faulds

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

Original languageEnglish
Pages (from-to)9412-9414
Number of pages3
JournalChemical Communications
Volume48
Issue number75
DOIs
Publication statusPublished - 2012

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Keywords

  • surface enhanced Raman scattering
  • DNA
  • TaqMan assay
  • SERS

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