Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Mhairi M. Harper; Barry Robertson; Alastair Ricketts; Karen Faulds

doi:10.1039/c2cc34859d

Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Mhairi M. Harper, Barry Robertson, Alastair Ricketts, Karen Faulds

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

Original language	English
Pages (from-to)	9412-9414
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	75
DOIs	https://doi.org/10.1039/c2cc34859d
Publication status	Published - 2012

Keywords

surface enhanced Raman scattering
DNA
TaqMan assay
SERS

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10.1039/c2cc34859d

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1 Finished

EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY
Faulds, K.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/07 → 31/10/11
Project: Research

Cite this

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abstract = "We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.",

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AU - Robertson, Barry

AU - Ricketts, Alastair

AU - Faulds, Karen

PY - 2012

Y1 - 2012

N2 - We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

AB - We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

KW - surface enhanced Raman scattering

KW - DNA

KW - TaqMan assay

KW - SERS

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JF - Chemical Communications

SN - 1359-7345

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Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

Abstract

Keywords

Access to Document

EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY

Cite this