Abstract
We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.
| Language | English |
|---|---|
| Pages | 9412-9414 |
| Number of pages | 3 |
| Journal | Chemical Communications |
| Volume | 48 |
| Issue number | 75 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- surface enhanced Raman scattering
- DNA
- TaqMan assay
- SERS
Cite this
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Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS). / Harper, Mhairi M.; Robertson, Barry; Ricketts, Alastair; Faulds, Karen.
In: Chemical Communications, Vol. 48, No. 75, 2012, p. 9412-9414.Research output: Contribution to journal › Article
TY - JOUR
T1 - Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)
AU - Harper, Mhairi M.
AU - Robertson, Barry
AU - Ricketts, Alastair
AU - Faulds, Karen
PY - 2012
Y1 - 2012
N2 - We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.
AB - We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.
KW - surface enhanced Raman scattering
KW - DNA
KW - TaqMan assay
KW - SERS
UR - http://www.scopus.com/inward/record.url?scp=84865206843&partnerID=8YFLogxK
U2 - 10.1039/c2cc34859d
DO - 10.1039/c2cc34859d
M3 - Article
VL - 48
SP - 9412
EP - 9414
JO - Chemical Communications
T2 - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 75
ER -