Species identification using the cytochrome b gene of commercial turtle shells

J.C.I. Lee, L.C. Tsai, S.P. Liao, A. Linacre, H.M. Hsieh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Turtle shells and their gelled products are familiar in some countries as foods, tonics and medicines. These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. We report on the design of five combinations of primer pairs for the identification of turtle shells and shell fragments used as ornaments, food products and medicines. The types of samples used are those encountered frequently and will typically contain highly degraded DNA. The success rate for species identification using the test described is dependent upon the choice of primer sets used and the length of the expected amplification product. Gelled products were simulated by the process of decoction for up to 12 h, after which all the turtle species could be identified from the liquid samples. This study establishes a method for the identification of commercial turtle shells and illustrates a simulated case using gelled products.
LanguageEnglish
Pages67-73
Number of pages6
JournalForensic Science International: Genetics
Volume3
Issue number2
DOIs
Publication statusPublished - Mar 2009

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Cytochromes b
Turtles
Genes
Endangered Species
Food
Legislation
DNA

Keywords

  • turtle shell
  • gelled product
  • cytochrome b
  • simulation test

Cite this

Lee, J.C.I. ; Tsai, L.C. ; Liao, S.P. ; Linacre, A. ; Hsieh, H.M. / Species identification using the cytochrome b gene of commercial turtle shells. In: Forensic Science International: Genetics . 2009 ; Vol. 3, No. 2. pp. 67-73.
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Species identification using the cytochrome b gene of commercial turtle shells. / Lee, J.C.I.; Tsai, L.C.; Liao, S.P.; Linacre, A.; Hsieh, H.M.

In: Forensic Science International: Genetics , Vol. 3, No. 2, 03.2009, p. 67-73.

Research output: Contribution to journalArticle

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