Species identification of rhinoceros horn using the cytochrome b gene

A.M.T. Linacre, H. Hsieh, L. Huang, L. Tsai, Y. Kuo, H. Meng, J.C. Lee

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Material suspected of originating from species of Rhinoceros is frequently seized by forensic organizations investigating trade in endangered species. At present identification of the species is possible by DNA sequencing of the material, such as powdered rhinoceros horns. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. The maximum value of genetic distance among white rhinoceros was 0.0176, and 0.0333 among black rhinoceros. In the comparison among rhinoceros species, the greatest genetic distance was between black and Indian rhinoceros (0.1564). The rhinoceros sequences extracted from GenBank and 13 samples in this study were clustered and separated from other mammals. Holstein cow was used as an out-group and was clustered with cattle in the phylogenetic tree. The results of this phylogenetic study also showed that there were four major branches among rhinoceros species from a common origin. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. In the initial identification of species from unknown powdered material, all the unknown samples were found to be from rhinoceroses. In phylogenetic analysis, the results supported the morphological hypothesis. The method used in this study can be applied in the identification of processed products of rhinoceros horns, such as sculptures, daggers, powders or even mixture powdered prescriptions.
LanguageEnglish
Pages1-11
Number of pages11
JournalForensic Science International
Volume136
Issue number1-3
DOIs
Publication statusPublished - 2003

Fingerprint

Cytochromes b
Horns
Nucleic Acid Databases
Sculpture
Genes
Endangered Species
DNA
DNA Sequence Analysis
Powders
Prescriptions
Mammals

Keywords

  • rhinoceros horn
  • cytochrome b gene

Cite this

Linacre, A. M. T., Hsieh, H., Huang, L., Tsai, L., Kuo, Y., Meng, H., & Lee, J. C. (2003). Species identification of rhinoceros horn using the cytochrome b gene. Forensic Science International, 136(1-3), 1-11. https://doi.org/10.1016/S0379-0738(03)00251-2
Linacre, A.M.T. ; Hsieh, H. ; Huang, L. ; Tsai, L. ; Kuo, Y. ; Meng, H. ; Lee, J.C. / Species identification of rhinoceros horn using the cytochrome b gene. In: Forensic Science International. 2003 ; Vol. 136, No. 1-3. pp. 1-11.
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Linacre, AMT, Hsieh, H, Huang, L, Tsai, L, Kuo, Y, Meng, H & Lee, JC 2003, 'Species identification of rhinoceros horn using the cytochrome b gene' Forensic Science International, vol. 136, no. 1-3, pp. 1-11. https://doi.org/10.1016/S0379-0738(03)00251-2

Species identification of rhinoceros horn using the cytochrome b gene. / Linacre, A.M.T.; Hsieh, H.; Huang, L.; Tsai, L.; Kuo, Y.; Meng, H.; Lee, J.C.

In: Forensic Science International, Vol. 136, No. 1-3, 2003, p. 1-11.

Research output: Contribution to journalArticle

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T1 - Species identification of rhinoceros horn using the cytochrome b gene

AU - Linacre, A.M.T.

AU - Hsieh, H.

AU - Huang, L.

AU - Tsai, L.

AU - Kuo, Y.

AU - Meng, H.

AU - Lee, J.C.

PY - 2003

Y1 - 2003

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AB - Material suspected of originating from species of Rhinoceros is frequently seized by forensic organizations investigating trade in endangered species. At present identification of the species is possible by DNA sequencing of the material, such as powdered rhinoceros horns. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. The maximum value of genetic distance among white rhinoceros was 0.0176, and 0.0333 among black rhinoceros. In the comparison among rhinoceros species, the greatest genetic distance was between black and Indian rhinoceros (0.1564). The rhinoceros sequences extracted from GenBank and 13 samples in this study were clustered and separated from other mammals. Holstein cow was used as an out-group and was clustered with cattle in the phylogenetic tree. The results of this phylogenetic study also showed that there were four major branches among rhinoceros species from a common origin. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. In the initial identification of species from unknown powdered material, all the unknown samples were found to be from rhinoceroses. In phylogenetic analysis, the results supported the morphological hypothesis. The method used in this study can be applied in the identification of processed products of rhinoceros horns, such as sculptures, daggers, powders or even mixture powdered prescriptions.

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EP - 11

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SN - 0379-0738

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