Solution conformation of an ETB selective agonist, ET-1 [Cys (Acm) 1, 15, Ala3, Leu7, Aib11], in CD3OH/H2O by 1H NMR and molecular modelling

Chandralal M Hewage, Lu Jiang, John A Parkinson, Robert Ramage, Ian H Sadler

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4 Citations (Scopus)

Abstract

To understand the basic structural requirements for the biological activity of endothelin peptides, the solution structure of an ETB selective agonist, ET-1[Cys(Acm)1,15,Ala3,Leu7,Aib11], was investigated by 1H NMR spectroscopy and molecular modelling. The structure is characterised by an α-helical conformation between residues Ser5-His16 but is undefined at both the N and C termini. To date, neither the solution structures of linear modified peptides nor the effects of a methanol/water solvent system have been examined for endothelin or endothelin-like peptides. This structure plays an important role towards the design of endothelin receptor selective agonists and antagonists.
Original languageEnglish
Pages (from-to)234-238
Number of pages5
JournalFEBS Letters
Volume425
Issue number2
DOIs
Publication statusPublished - 27 Mar 1998

Keywords

  • endothelin
  • molecular modelling
  • nuclear magnetic resonance
  • peptide solution structure

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