siRNA delivery to melanoma cells with cationic niosomes

Mohammad A. Obeid, Hanin Alyamani, Haneen Amawi, Alaa A. A. Aljabali, Meriem Rezigue, Shatha N. Abdeljaber, Valerie A. Ferro

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Citations (Scopus)

Abstract

RNA interference (RNAi) is a posttranscriptional regulatory mechanism that employs siRNA. It typically results in the degradation of a target mRNA that encodes a particular protein. Treatment with siRNA therapeutics requires the use of an effective drug delivery system to assist in delivering these therapeutics into the cytoplasm of the transfected cells. Here we describe the transfection of melanoma cancer cells with siRNA using cationic niosome nanoparticles as a delivery system. The method of niosome preparation is first introduced and is followed by complex formation with siRNA and the transfection method.
Original languageEnglish
Title of host publicationMelanoma
EditorsK.M. Hargadon
Place of PublicationNew York
Pages621-634
Number of pages14
ISBN (Electronic)9781071612057
DOIs
Publication statusE-pub ahead of print - 12 Mar 2021

Publication series

NameMethods in Molecular Biology
PublisherSpringer Verlag
Volume2265
ISSN (Print)1064-3745

Keywords

  • niosome nanoparticles
  • microfluidic mixing
  • thin-film hydration method
  • siRNA
  • cellular transfection
  • cytotoxicity

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