Single-shot time-gated fluorescence lifetime imaging using three-frame images

Yahui Li, Hui Jia, Shaorong Chen, Jinshou Tian, Lingliang Liang, Fenfang Yuan, Hongqi Yu, David Li

Research output: Contribution to journalArticle

Abstract

Qualitative and quantitative measurements of complex flows demand for fast single-shot fluorescence lifetime imaging (FLI) technology with high precision. A method, single-shot time-gated fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is presented. To our knowledge, it is the first work to combine a three-gate rapid lifetime determination (RLD) scheme and a four-channel framing camera to achieve this goal. Different from previously proposed two-gate RLD schemes, TFI-TGFLI can provide a wider lifetime range 0.6 ~ 13ns with reasonable precision. The performances of the proposed approach have been examined by both Monte-Carlo simulations and toluene seeded gas mixing jet diagnosis experiments. The measured average lifetimes of the whole excited areas agree well with the results obtained by the streak camera, and they are 7.6ns (N2 = 7L/min; O2 < 0.1L/min) and 2.6ns (N2 = 19L/min; O2 = 1L/min) with the standard deviations of 1.7ns and 0.8ns among the lifetime image pixels, respectively. The concentration distributions of the quenchers and fluorescent species were further analyzed, and they are consistent with the experimental settings.
LanguageEnglish
Pages17936-17947
Number of pages12
JournalOptics Express
Volume26
Issue number4
DOIs
Publication statusPublished - 27 Jun 2018

Fingerprint

shot
life (durability)
fluorescence
framing cameras
streak cameras
toluene
standard deviation
pixels
gases
simulation

Keywords

  • fluorescence lifetime imaging
  • rapid lifetime determination
  • single-shot
  • time-gated
  • four-channel framing camera
  • TGFLI

Cite this

Li, Yahui ; Jia, Hui ; Chen, Shaorong ; Tian, Jinshou ; Liang, Lingliang ; Yuan, Fenfang ; Yu, Hongqi ; Li, David. / Single-shot time-gated fluorescence lifetime imaging using three-frame images. In: Optics Express. 2018 ; Vol. 26, No. 4. pp. 17936-17947.
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abstract = "Qualitative and quantitative measurements of complex flows demand for fast single-shot fluorescence lifetime imaging (FLI) technology with high precision. A method, single-shot time-gated fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is presented. To our knowledge, it is the first work to combine a three-gate rapid lifetime determination (RLD) scheme and a four-channel framing camera to achieve this goal. Different from previously proposed two-gate RLD schemes, TFI-TGFLI can provide a wider lifetime range 0.6 ~ 13ns with reasonable precision. The performances of the proposed approach have been examined by both Monte-Carlo simulations and toluene seeded gas mixing jet diagnosis experiments. The measured average lifetimes of the whole excited areas agree well with the results obtained by the streak camera, and they are 7.6ns (N2 = 7L/min; O2 < 0.1L/min) and 2.6ns (N2 = 19L/min; O2 = 1L/min) with the standard deviations of 1.7ns and 0.8ns among the lifetime image pixels, respectively. The concentration distributions of the quenchers and fluorescent species were further analyzed, and they are consistent with the experimental settings.",
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Li, Y, Jia, H, Chen, S, Tian, J, Liang, L, Yuan, F, Yu, H & Li, D 2018, 'Single-shot time-gated fluorescence lifetime imaging using three-frame images' Optics Express, vol. 26, no. 4, pp. 17936-17947. https://doi.org/10.1364/OE.26.017936

Single-shot time-gated fluorescence lifetime imaging using three-frame images. / Li, Yahui; Jia, Hui; Chen, Shaorong; Tian, Jinshou; Liang, Lingliang; Yuan, Fenfang; Yu, Hongqi; Li, David.

In: Optics Express, Vol. 26, No. 4, 27.06.2018, p. 17936-17947.

Research output: Contribution to journalArticle

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Li Y, Jia H, Chen S, Tian J, Liang L, Yuan F et al. Single-shot time-gated fluorescence lifetime imaging using three-frame images. Optics Express. 2018 Jun 27;26(4):17936-17947. https://doi.org/10.1364/OE.26.017936