Abstract
ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy. It is open-source software, platform-independent and enables students and researchers to obtain an easy but thorough introduction into image analysis. Especially the image processing package Fiji is a valuable and powerful extension of ImageJ. Several plugins and macros for single-molecule localization microscopy (SMLM) have been developed during the last decade. These novel tools cover the steps from single-molecule localization and image reconstruction to SMLM data postprocessing such as density analysis, image registration or resolution estimation. This article describes how ImageJ/Fiji can be used for image analysis, reviews existing extensions for SMLM, and aims at introducing and motivating novices and advanced SMLM users alike to explore the possibilities of ImageJ/Fiji for automated and quantitative data analysis.
Original language | English |
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Article number | 203002 |
Number of pages | 11 |
Journal | Journal of Physics D: Applied Physics |
Volume | 52 |
Issue number | 20 |
Early online date | 21 Feb 2019 |
DOIs | |
Publication status | Published - 13 Mar 2019 |
Keywords
- ImageJ
- Fiji
- image analysis
- super-resolution
- quantitative imaging
- SMLM