Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering

C.D McGuinness, A.M. Macmillan, J. Karolin, W.E. Smith, D. Graham, J.C. Pickup, D.J.S. Birch

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm-1 band was 1 × 10-13 mol l-1, compared to 2 × 10-12 mol l-1 for the fluorescence peak at 660 nm.
LanguageEnglish
Pages633-634
Number of pages1
JournalAnalyst
Volume132
Issue number7
DOIs
Publication statusPublished - 2007

Fingerprint

Raman Spectrum Analysis
Limit of Detection
Raman scattering
Fluorescence
scattering
Infrared radiation
Proteins
Molecules
near infrared
fluorescence
protein
allophycocyanin
detection

Keywords

  • surface enhanced resonance Raman scattering
  • SERRS
  • allophycocyanin
  • single molecule level detection

Cite this

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title = "Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering",
abstract = "Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm-1 band was 1 × 10-13 mol l-1, compared to 2 × 10-12 mol l-1 for the fluorescence peak at 660 nm.",
keywords = "surface enhanced resonance Raman scattering, SERRS, allophycocyanin, single molecule level detection",
author = "C.D McGuinness and A.M. Macmillan and J. Karolin and W.E. Smith and D. Graham and J.C. Pickup and D.J.S. Birch",
year = "2007",
doi = "10.1039/b706409h",
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pages = "633--634",
journal = "Analyst",
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Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering. / McGuinness, C.D; Macmillan, A.M.; Karolin, J.; Smith, W.E.; Graham, D.; Pickup, J.C.; Birch, D.J.S.

In: Analyst, Vol. 132, No. 7, 2007, p. 633-634.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering

AU - McGuinness, C.D

AU - Macmillan, A.M.

AU - Karolin, J.

AU - Smith, W.E.

AU - Graham, D.

AU - Pickup, J.C.

AU - Birch, D.J.S.

PY - 2007

Y1 - 2007

N2 - Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm-1 band was 1 × 10-13 mol l-1, compared to 2 × 10-12 mol l-1 for the fluorescence peak at 660 nm.

AB - Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm-1 band was 1 × 10-13 mol l-1, compared to 2 × 10-12 mol l-1 for the fluorescence peak at 660 nm.

KW - surface enhanced resonance Raman scattering

KW - SERRS

KW - allophycocyanin

KW - single molecule level detection

UR - http://dx.doi.org/10.1039/b706409h

U2 - 10.1039/b706409h

DO - 10.1039/b706409h

M3 - Article

VL - 132

SP - 633

EP - 634

JO - Analyst

T2 - Analyst

JF - Analyst

SN - 0003-2654

IS - 7

ER -