Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

F. McKenzie; R.J. Stokes; K. Faulds; D. Graham

doi:10.1117/12.794965

Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

F. McKenzie, R.J. Stokes, K. Faulds, D. Graham

Pure And Applied Chemistry

Research output: Contribution to journal › Conference article › peer-review

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Abstract

Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

Original language	English
Pages (from-to)	J350-J350
Journal	Proceedings of SPIE
Volume	7035
DOIs	https://doi.org/10.1117/12.794965
Publication status	Published - 29 Aug 2008
Event	Biosensing - San Diego, CA, USA Duration: 12 Aug 2008 → …

Keywords

gold nanoparticles
silver nanoparticles
DNA
locked nucleic acid
LNA

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10.1117/12.794965

70350J
Copyright © 2008 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D. (Principal Investigator), Dawson, M. (Co-investigator), Faulds, K. (Co-investigator), Girkin, J. (Co-investigator), Graham, D. (Co-investigator), Martin, R. (Co-investigator), O'Donnell, K. (Co-investigator), Rolinski, O. (Co-investigator), Smith, W. (Co-investigator) & Wynne, K. (Co-investigator)
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research

Cite this

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title = "Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles",

abstract = "Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.",

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AU - McKenzie, F.

AU - Stokes, R.J.

AU - Faulds, K.

AU - Graham, D.

PY - 2008/8/29

Y1 - 2008/8/29

N2 - Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

AB - Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

KW - gold nanoparticles

KW - silver nanoparticles

KW - DNA

KW - locked nucleic acid

KW - LNA

U2 - 10.1117/12.794965

DO - 10.1117/12.794965

M3 - Conference article

SN - 0277-786X

VL - 7035

SP - J350-J350

JO - Proceedings of SPIE

JF - Proceedings of SPIE

T2 - Biosensing

Y2 - 12 August 2008

ER -

Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

Abstract

Keywords

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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

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