Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

F. McKenzie, R.J. Stokes, K. Faulds, D. Graham

Research output: Contribution to conferencePaper

Abstract

Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.
LanguageEnglish
PagesJ350-J350
Publication statusPublished - 29 Aug 2008
EventBiosensing - San Diego, CA, USA
Duration: 12 Aug 2008 → …

Conference

ConferenceBiosensing
CitySan Diego, CA, USA
Period12/08/08 → …

Fingerprint

Single-Stranded DNA
Nanoparticles
DNA
Gold
DNA Probes
Silver
Oligonucleotides
Raman scattering
Labels
locked nucleic acid

Keywords

  • gold nanoparticles
  • silver nanoparticles
  • DNA
  • locked nucleic acid
  • LNA

Cite this

McKenzie, F., Stokes, R. J., Faulds, K., & Graham, D. (2008). Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles. J350-J350. Paper presented at Biosensing, San Diego, CA, USA, .
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McKenzie, F, Stokes, RJ, Faulds, K & Graham, D 2008, 'Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles' Paper presented at Biosensing, San Diego, CA, USA, 12/08/08, pp. J350-J350.

Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles. / McKenzie, F.; Stokes, R.J.; Faulds, K.; Graham, D.

2008. J350-J350 Paper presented at Biosensing, San Diego, CA, USA, .

Research output: Contribution to conferencePaper

TY - CONF

T1 - Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

AU - McKenzie, F.

AU - Stokes, R.J.

AU - Faulds, K.

AU - Graham, D.

PY - 2008/8/29

Y1 - 2008/8/29

N2 - Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

AB - Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

KW - gold nanoparticles

KW - silver nanoparticles

KW - DNA

KW - locked nucleic acid

KW - LNA

UR - http://dx.doi.org/10.1117/12.794965

M3 - Paper

SP - J350-J350

ER -

McKenzie F, Stokes RJ, Faulds K, Graham D. Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles. 2008. Paper presented at Biosensing, San Diego, CA, USA, .