Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal

B. Somasundaram; J. Hoyland; John Dempster; J. Riley; W.T. Mason

Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal

B. Somasundaram, J. Hoyland, John Dempster, J. Riley, W.T. Mason

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Conference Contribution › peer-review

Abstract

This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged
directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second.

Original language	English
Pages (from-to)	P63-P63
Number of pages	1
Journal	Journal of Physiology
Volume	446
Issue number	Suppl.
Publication status	Published - 1992
Event	The Physiological Society Cambridge Meeting 1991 - Cambridge, United Kingdom Duration: 18 Jul 1991 → 20 Jul 1991

Keywords

patch clamp
photometric fluorescence
PhoCal

Cite this

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title = "Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal",

abstract = "This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second. ",

keywords = "patch clamp, photometric fluorescence, PhoCal",

author = "B. Somasundaram and J. Hoyland and John Dempster and J. Riley and W.T. Mason",

year = "1992",

language = "English",

volume = "446",

pages = "P63--P63",

journal = "Journal of Physiology",

issn = "0022-3751",

number = "Suppl.",

note = "The Physiological Society Cambridge Meeting 1991 ; Conference date: 18-07-1991 Through 20-07-1991",

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TY - JOUR

T1 - Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal

AU - Somasundaram, B.

AU - Hoyland, J.

AU - Dempster, John

AU - Riley, J.

AU - Mason, W.T.

PY - 1992

Y1 - 1992

N2 - This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second.

AB - This demonstration will present a new computer program and analytical system - PhoCal - which permits the simultaneous measurement of (a) low light levels of fluorescence from optical probes for ions and (b) up to two channels of physiological data which can include patch-clamp current and voltage, or voltage from other probes such as extracellular pH electrodes or other transducers and (c) an event marker channel. The system is PC-based and uses high speed analogto-digital circuitry operating in parallel with a two-channel photon counter which collects data from one or two photomultiplier tubes. All data is logged directly to hard disk and the system can operate at speeds in excess of an aggregate of 1000 samples per second.

KW - patch clamp

KW - photometric fluorescence

KW - PhoCal

UR - http://jp.physoc.org/content/446/1/59P.full.pdf+html

M3 - Conference Contribution

SN - 0022-3751

VL - 446

SP - P63-P63

JO - Journal of Physiology

JF - Journal of Physiology

IS - Suppl.

T2 - The Physiological Society Cambridge Meeting 1991

Y2 - 18 July 1991 through 20 July 1991

ER -

Simultaneous patch-clamp and photometric fluorescence ratio measurements of optical probes for ions using a computer-based data acquisition system, PhoCal

Abstract

Keywords

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