Simultaneous determination of glycerol and clavulanic acid in an antibiotic process using attenuated total reflectance mid infrared spectroscopy

P. Roychoudhury, B. McNeil, L.M. Harvey

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Attenuated total reflectance mid infrared (ATR-MIR) spectroscopy is a potential technique for the near real-time monitoring of filamentous bioprocesses. Here we investigate the utility of ATR-MIR to monitor and predict concentrations of glycerol and product (clavulanic acid) in a complex antibiotic bioprocess. Streptomyces clavuligerus exhibits filamentous growth, thus, as biomass accumulates the process fluid becomes much more viscous, and develops pronounced non-Newtonian behaviour. A multivariate statistical technique, partial least square (PLS) has been used to develop models for the key analytes over the time course of the bioprocess. These models were then validated externally using unseen samples, not used in the original modelling exercise. Despite the heterogeneous nature of the bioprocess and the resulting complexity of the spectra, the models developed had high correlation coefficient values and low prediction error values of 0.302 and 0.009 for glycerol and clavulanic acid, respectively. The findings extend the use of ATR-MIR in these difficult fluids which are typical of filamentous industrial bioprocesses, and demonstrate the practical utility of the technique in the measurement of a range of analyte types, including those present at relatively modest levels compared to the concentrations of biomass and major substrates.
LanguageEnglish
Pages246-252
Number of pages7
JournalAnalytica Chimica Acta
Volume585
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Clavulanic Acid
infrared spectroscopy
antibiotics
Biomass
Glycerol
Infrared spectroscopy
Spectrum Analysis
reflectance
Anti-Bacterial Agents
acid
Streptomyces
Least-Squares Analysis
Infrared radiation
Fluids
fluid
biomass
Growth
substrate
Monitoring
Substrates

Keywords

  • attenuated total reflectance mid infrared spectroscopy;
  • streptomyces
  • multivariate statistical technique
  • partial least squares

Cite this

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title = "Simultaneous determination of glycerol and clavulanic acid in an antibiotic process using attenuated total reflectance mid infrared spectroscopy",
abstract = "Attenuated total reflectance mid infrared (ATR-MIR) spectroscopy is a potential technique for the near real-time monitoring of filamentous bioprocesses. Here we investigate the utility of ATR-MIR to monitor and predict concentrations of glycerol and product (clavulanic acid) in a complex antibiotic bioprocess. Streptomyces clavuligerus exhibits filamentous growth, thus, as biomass accumulates the process fluid becomes much more viscous, and develops pronounced non-Newtonian behaviour. A multivariate statistical technique, partial least square (PLS) has been used to develop models for the key analytes over the time course of the bioprocess. These models were then validated externally using unseen samples, not used in the original modelling exercise. Despite the heterogeneous nature of the bioprocess and the resulting complexity of the spectra, the models developed had high correlation coefficient values and low prediction error values of 0.302 and 0.009 for glycerol and clavulanic acid, respectively. The findings extend the use of ATR-MIR in these difficult fluids which are typical of filamentous industrial bioprocesses, and demonstrate the practical utility of the technique in the measurement of a range of analyte types, including those present at relatively modest levels compared to the concentrations of biomass and major substrates.",
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Simultaneous determination of glycerol and clavulanic acid in an antibiotic process using attenuated total reflectance mid infrared spectroscopy. / Roychoudhury, P.; McNeil, B.; Harvey, L.M.

In: Analytica Chimica Acta, Vol. 585, No. 2, 2007, p. 246-252.

Research output: Contribution to journalArticle

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