Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS

A.M. Ingram; B.D. Moore; D. Graham

doi:10.1016/j.bmcl.2009.02.030

Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS

A.M. Ingram, B.D. Moore, D. Graham

Pure And Applied Chemistry

Research output: Contribution to journal › Article

59 Citations (Scopus)

Abstract

Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.

Original language	English
Pages (from-to)	1569-1571
Number of pages	2
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	6
DOIs	https://doi.org/10.1016/j.bmcl.2009.02.030
Publication status	Published - 15 Mar 2009

Keywords

enzymes
surface enhanced Raman scattering

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10.1016/j.bmcl.2009.02.030

http://dx.doi.org/10.1016/j.bmcl.2009.02.030

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Ingram, A. M., Moore, B. D., & Graham, D. (2009). Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS. Bioorganic and Medicinal Chemistry Letters, 19(6), 1569-1571. https://doi.org/10.1016/j.bmcl.2009.02.030

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abstract = "Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.",

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AB - Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.

KW - enzymes

KW - surface enhanced Raman scattering

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