Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS

A.M. Ingram, B.D. Moore, D. Graham

Research output: Contribution to journalArticle

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Abstract

Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.
LanguageEnglish
Pages1569-1571
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number6
DOIs
Publication statusPublished - 15 Mar 2009

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Raman Spectrum Analysis
beta-Galactosidase
Alkaline Phosphatase
Raman scattering
Thermodynamic properties
Lipase
Esters
Azo Compounds
Acylation
Silver
Nanoparticles
Hydrolysis
Coloring Agents
Fluorescence
Derivatives

Keywords

  • enzymes
  • surface enhanced Raman scattering

Cite this

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Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS. / Ingram, A.M.; Moore, B.D.; Graham, D.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 19, No. 6, 15.03.2009, p. 1569-1571.

Research output: Contribution to journalArticle

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AU - Graham, D.

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