Silver colloids as plasmonic substrates for direct label-free surface-enhanced Raman scattering analysis of DNA

A. Torres-Nuñez, K. Faulds, D. Graham, R. A. Alvarez-Puebla, L. Guerrini

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Ultrasensitive direct SERS analysis offers a powerful analytical tool for the structural characterization and classification of nucleic acids. However, acquisition of reliable spectral fingerprints of such complex biomolecules poses important challenges. In recent years, many efforts have been devoted to overcome these limitations, mainly implementing silver colloids as plasmonic substrates. However, a reliable cross-comparison of results reported in the recent literature is extremely hard to achieve, mostly due to the broad set of different surface properties of the plasmonic nanoparticles. Herein, we perform a thorough investigation of the role played by the metal/liquid interface composition of silver colloids in the direct label-free SERS analysis of DNA. Target molecules of increasing complexity, from short homopolymeric strands to long genomic duplexes, were used as probes. We demonstrate how apparently subtle changes in the colloidal surface chemistry can dramatically modify the affinity and the final SERS spectral profile of DNA. This has significant implications for the future design of new analytical strategies for the detection of DNA using SERS without labels.

LanguageEnglish
Pages5170-5180
Number of pages11
JournalAnalyst
Volume141
Issue number17
Early online date12 May 2016
DOIs
Publication statusPublished - 7 Sep 2016

Fingerprint

Raman Spectrum Analysis
colloid
Colloids
Raman scattering
Labels
silver
Silver
DNA
scattering
substrate
Substrates
Surface Properties
duplex
Nucleic acids
Dermatoglyphics
Biomolecules
nucleic acid
Surface chemistry
Liquid metals
Nanoparticles

Keywords

  • silver colloids
  • Raman scattering
  • plasmonic substrates
  • DNA

Cite this

Torres-Nuñez, A. ; Faulds, K. ; Graham, D. ; Alvarez-Puebla, R. A. ; Guerrini, L. / Silver colloids as plasmonic substrates for direct label-free surface-enhanced Raman scattering analysis of DNA. In: Analyst. 2016 ; Vol. 141, No. 17. pp. 5170-5180.
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Silver colloids as plasmonic substrates for direct label-free surface-enhanced Raman scattering analysis of DNA. / Torres-Nuñez, A.; Faulds, K.; Graham, D.; Alvarez-Puebla, R. A.; Guerrini, L.

In: Analyst, Vol. 141, No. 17, 07.09.2016, p. 5170-5180.

Research output: Contribution to journalArticle

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