Silica condensation by a silicatein alpha homologue involves surface-induced transition to a stable structural intermediate forming a saturated monolayer

Siddharth V. Patwardhan, Stephen A. Holt, Sharon M. Kelly, Michaela Kreiner, Carole C. Perry, Christopher F. van der Walle

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Silicatein alpha exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly beta-sheet structure. However, it is produced in a soluble form with mixed alpha-helix/beta-sheet structure akin to its cathepsin L homologue. To understand this conformational transition in the context of enzyme catalyzed silica condensation, we used a functional, recombinant silicatein alpha termed 4SER. In solution, 4SER becomes conformationally unstable at pH 7 and readily unfolds to a soluble beta-sheet intermediate, losing the majority of its helical structure. This beta-sheet intermediate is present following adsorption of 4SER to a silica surface from solution. 4SER is particularly surface active, forming a near saturated monolayer on SiO2 from low bulk concentrations, without transition to multilayers at high bulk concentrations. The adsorbed intermediate remains stable during silica condensation and drying. We propose that the beta-sheet structure for silicatein a. in marine sponge spicules represents a stable structural intermediate, formed upon adsorption to the silica surface.

LanguageEnglish
Pages3126-3135
Number of pages10
JournalBiomacromolecules
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Silicon Dioxide
Condensation
Monolayers
Silica
Cathepsin L
Adsorption
Drying
Multilayers
Enzymes
silicatein alpha
Proteins

Keywords

  • protein secondary structures
  • demosponge suberites-dumuncula
  • human procathepsin l
  • circular-dichroism
  • conformational stability
  • enzyme immobilization
  • in-virto
  • adsorption
  • spicules

Cite this

Patwardhan, Siddharth V. ; Holt, Stephen A. ; Kelly, Sharon M. ; Kreiner, Michaela ; Perry, Carole C. ; van der Walle, Christopher F. / Silica condensation by a silicatein alpha homologue involves surface-induced transition to a stable structural intermediate forming a saturated monolayer. In: Biomacromolecules. 2010 ; Vol. 11, No. 11. pp. 3126-3135.
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Silica condensation by a silicatein alpha homologue involves surface-induced transition to a stable structural intermediate forming a saturated monolayer. / Patwardhan, Siddharth V.; Holt, Stephen A.; Kelly, Sharon M.; Kreiner, Michaela; Perry, Carole C.; van der Walle, Christopher F.

In: Biomacromolecules, Vol. 11, No. 11, 11.2010, p. 3126-3135.

Research output: Contribution to journalArticle

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