Sheep mast cell proteinase-1 (sMCP-1), a serine proteinase with dual chymase/tryptase activity, is expressed in gastrointestinal mast cells, and released systemically and on to the mucosal surface during gastrointestinal nematode infection. The potential for native plasma proteinase inhibitors to control sMCP-1 activity was investigated. Sheep α1-proteinase inhibitor (α1PI) inhibited sMCP-1 slowly, with second-order association rate constant (k(ass)) 1.1 x 103 M-1 s-1, whereas sheep contrapsin inhibited trypsin (k(ass) 2.2 x 106 M-1 s-1) but not sMCP-1. Western-blot analysis and gel filtration showed that when added to serum or plasma, sMCP-1 was partitioned between α1PI and α2-macroglobulin. The possibility that significant cleavage of plasma proteins could occur before sMCP-1 was inhibited was investigated using gel filtration and SDS/PAGE after adding sMCP-1 to plasma. Cleavage of ovine fibrinogen occurred in the presence of excess α1PI and α2-macroglobulin, the α-chain being cleaved C-terminally and the β-chain at the putative Lys-27. In addition, sMCP-1 was found to be mitogenic for bovine pulmonary artery fibroblasts, but was not mitogenic in the presence of soya-bean trypsin inhibitor. In terms of fibrinogen cleavage and fibroblast stimulation, sMCP-1 shows functional similarities to mast cell tryptase.
- sheep mast cell proteinase-1
- serine proteinase
- plasma proteinase inhibitors
- bovine pulmonary artery fibroblasts