SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers

Haley Marks, Samuel Mabbott, George W. Jackson, Duncan Graham, Gerard L. Cote

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

2 Citations (Scopus)

Abstract

Functionalized colloidal nanoparticles for SERS serve as a promising multifunctional assay component for blood biomarker detection. Proper design of these nanoprobes through conjugation to spectral tags, protective polymers, and sensing ligands can provide experimental control over the sensitivity, range, reproducibility, particle stability, and integration with biorecognition assays. Additionally, the optical properties and degree of electromagnetic SERS signal enhancement can be altered and monitored through tuning the nanoparticle shape, size, material and the colloid's local surface plasmon resonance (LSPR). Aptamers, synthetic affinity ligands derived from nucleic acids, provide a number of advantages for biorecognition of small molecules and toxins with low immunogenicity. DNA aptamers are simpler and more economical to produce at large scale, are capable of greater specificity and affinity than antibodies, are easily tailored to specific functional groups, can be used to tune inter-particle distance and shift the LSPR, and their intrinsic negative charge can be utilized for additional particle stability.<sup>1,2</sup> Herein, a "turn-off" competitive binding assay platform involving two different plasmonic nanoparticles for the detection of the toxin bisphenol A (BPA) using SERS is presented. A derivative of the toxin is immobilized onto a silver coated magnetic nanoparticle (Ag@MNP), and a second solid silver nanoparticle (AgNP) is functionalized with the BPA aptamer and a Raman reporter molecule (RRM). The capture (Ag@MNP) and probe (AgNP) particles are mixed and the aptamer binding interaction draws the nanoparticles closer together, forming an assembly that results in an increased SERS signal intensity. This aptamer mediated assembly of the two nanoparticles results in a 100x enhancement of the SERS signal intensity from the RRM. These pre-bound aptamer/nanoparticle conjugates were then exposed to BPA in free solution and the competitive binding event was monitored by the decrease in SERS intensity.

LanguageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsWolfgang J. Parak, Marek Osinski, Xing-Jie Liang
Place of PublicationSan Francisco
Number of pages5
DOIs
Publication statusPublished - 12 Mar 2015
EventColloidal Nanoparticles for Biomedical Applications X - San Francisco, United States
Duration: 7 Feb 20159 Feb 2015

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume9338
ISSN (Print)0277-786X

Conference

ConferenceColloidal Nanoparticles for Biomedical Applications X
CountryUnited States
CitySan Francisco
Period7/02/159/02/15

Fingerprint

biomarkers
Biomarkers
Nanoparticles
blood
Blood
nanoparticles
bisphenols
Assays
Competitive Binding
Surface Plasmon Resonance
Surface plasmon resonance
Silver
surface plasmon resonance
Molecules
affinity
assembly
Ligands
silver
Nucleotide Aptamers
Nanoprobes

Keywords

  • aptamer
  • competitive binding assay
  • molecular diagnostics
  • plasmonic nanoparticles
  • Surface enhanced Raman spectroscopy (SERS)

Cite this

Marks, H., Mabbott, S., Jackson, G. W., Graham, D., & Cote, G. L. (2015). SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. In W. J. Parak, M. Osinski, & X-J. Liang (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE [93381C] (Proceedings of SPIE; Vol. 9338). San Francisco. https://doi.org/10.1117/12.2078869
Marks, Haley ; Mabbott, Samuel ; Jackson, George W. ; Graham, Duncan ; Cote, Gerard L. / SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / Wolfgang J. Parak ; Marek Osinski ; Xing-Jie Liang. San Francisco, 2015. (Proceedings of SPIE).
@inproceedings{2aa187d49e444e8ba3448b5d8785da24,
title = "SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers",
abstract = "Functionalized colloidal nanoparticles for SERS serve as a promising multifunctional assay component for blood biomarker detection. Proper design of these nanoprobes through conjugation to spectral tags, protective polymers, and sensing ligands can provide experimental control over the sensitivity, range, reproducibility, particle stability, and integration with biorecognition assays. Additionally, the optical properties and degree of electromagnetic SERS signal enhancement can be altered and monitored through tuning the nanoparticle shape, size, material and the colloid's local surface plasmon resonance (LSPR). Aptamers, synthetic affinity ligands derived from nucleic acids, provide a number of advantages for biorecognition of small molecules and toxins with low immunogenicity. DNA aptamers are simpler and more economical to produce at large scale, are capable of greater specificity and affinity than antibodies, are easily tailored to specific functional groups, can be used to tune inter-particle distance and shift the LSPR, and their intrinsic negative charge can be utilized for additional particle stability.1,2 Herein, a {"}turn-off{"} competitive binding assay platform involving two different plasmonic nanoparticles for the detection of the toxin bisphenol A (BPA) using SERS is presented. A derivative of the toxin is immobilized onto a silver coated magnetic nanoparticle (Ag@MNP), and a second solid silver nanoparticle (AgNP) is functionalized with the BPA aptamer and a Raman reporter molecule (RRM). The capture (Ag@MNP) and probe (AgNP) particles are mixed and the aptamer binding interaction draws the nanoparticles closer together, forming an assembly that results in an increased SERS signal intensity. This aptamer mediated assembly of the two nanoparticles results in a 100x enhancement of the SERS signal intensity from the RRM. These pre-bound aptamer/nanoparticle conjugates were then exposed to BPA in free solution and the competitive binding event was monitored by the decrease in SERS intensity.",
keywords = "aptamer, competitive binding assay, molecular diagnostics, plasmonic nanoparticles, Surface enhanced Raman spectroscopy (SERS)",
author = "Haley Marks and Samuel Mabbott and Jackson, {George W.} and Duncan Graham and Cote, {Gerard L.}",
note = "Marks, H., Mabbott, S., Jackson, G. W., Graham, D., & Cote, G. L. {"}SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers{"} Progress in Biomedical Optics and Imaging - Proceedings of SPIE. [93381C], W. J. Parak, M. Osinski, & X-J. Liang (Eds.), Vol. 9338, 93381C (2015). Copyright 2015 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2078869",
year = "2015",
month = "3",
day = "12",
doi = "10.1117/12.2078869",
language = "English",
isbn = "9781628414288",
series = "Proceedings of SPIE",
publisher = "SPIE",
editor = "Parak, {Wolfgang J.} and Marek Osinski and Xing-Jie Liang",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

Marks, H, Mabbott, S, Jackson, GW, Graham, D & Cote, GL 2015, SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. in WJ Parak, M Osinski & X-J Liang (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE., 93381C, Proceedings of SPIE, vol. 9338, San Francisco, Colloidal Nanoparticles for Biomedical Applications X, San Francisco, United States, 7/02/15. https://doi.org/10.1117/12.2078869

SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. / Marks, Haley; Mabbott, Samuel; Jackson, George W.; Graham, Duncan; Cote, Gerard L.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang. San Francisco, 2015. 93381C (Proceedings of SPIE; Vol. 9338).

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

T1 - SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers

AU - Marks, Haley

AU - Mabbott, Samuel

AU - Jackson, George W.

AU - Graham, Duncan

AU - Cote, Gerard L.

N1 - Marks, H., Mabbott, S., Jackson, G. W., Graham, D., & Cote, G. L. "SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers" Progress in Biomedical Optics and Imaging - Proceedings of SPIE. [93381C], W. J. Parak, M. Osinski, & X-J. Liang (Eds.), Vol. 9338, 93381C (2015). Copyright 2015 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2078869

PY - 2015/3/12

Y1 - 2015/3/12

N2 - Functionalized colloidal nanoparticles for SERS serve as a promising multifunctional assay component for blood biomarker detection. Proper design of these nanoprobes through conjugation to spectral tags, protective polymers, and sensing ligands can provide experimental control over the sensitivity, range, reproducibility, particle stability, and integration with biorecognition assays. Additionally, the optical properties and degree of electromagnetic SERS signal enhancement can be altered and monitored through tuning the nanoparticle shape, size, material and the colloid's local surface plasmon resonance (LSPR). Aptamers, synthetic affinity ligands derived from nucleic acids, provide a number of advantages for biorecognition of small molecules and toxins with low immunogenicity. DNA aptamers are simpler and more economical to produce at large scale, are capable of greater specificity and affinity than antibodies, are easily tailored to specific functional groups, can be used to tune inter-particle distance and shift the LSPR, and their intrinsic negative charge can be utilized for additional particle stability.1,2 Herein, a "turn-off" competitive binding assay platform involving two different plasmonic nanoparticles for the detection of the toxin bisphenol A (BPA) using SERS is presented. A derivative of the toxin is immobilized onto a silver coated magnetic nanoparticle (Ag@MNP), and a second solid silver nanoparticle (AgNP) is functionalized with the BPA aptamer and a Raman reporter molecule (RRM). The capture (Ag@MNP) and probe (AgNP) particles are mixed and the aptamer binding interaction draws the nanoparticles closer together, forming an assembly that results in an increased SERS signal intensity. This aptamer mediated assembly of the two nanoparticles results in a 100x enhancement of the SERS signal intensity from the RRM. These pre-bound aptamer/nanoparticle conjugates were then exposed to BPA in free solution and the competitive binding event was monitored by the decrease in SERS intensity.

AB - Functionalized colloidal nanoparticles for SERS serve as a promising multifunctional assay component for blood biomarker detection. Proper design of these nanoprobes through conjugation to spectral tags, protective polymers, and sensing ligands can provide experimental control over the sensitivity, range, reproducibility, particle stability, and integration with biorecognition assays. Additionally, the optical properties and degree of electromagnetic SERS signal enhancement can be altered and monitored through tuning the nanoparticle shape, size, material and the colloid's local surface plasmon resonance (LSPR). Aptamers, synthetic affinity ligands derived from nucleic acids, provide a number of advantages for biorecognition of small molecules and toxins with low immunogenicity. DNA aptamers are simpler and more economical to produce at large scale, are capable of greater specificity and affinity than antibodies, are easily tailored to specific functional groups, can be used to tune inter-particle distance and shift the LSPR, and their intrinsic negative charge can be utilized for additional particle stability.1,2 Herein, a "turn-off" competitive binding assay platform involving two different plasmonic nanoparticles for the detection of the toxin bisphenol A (BPA) using SERS is presented. A derivative of the toxin is immobilized onto a silver coated magnetic nanoparticle (Ag@MNP), and a second solid silver nanoparticle (AgNP) is functionalized with the BPA aptamer and a Raman reporter molecule (RRM). The capture (Ag@MNP) and probe (AgNP) particles are mixed and the aptamer binding interaction draws the nanoparticles closer together, forming an assembly that results in an increased SERS signal intensity. This aptamer mediated assembly of the two nanoparticles results in a 100x enhancement of the SERS signal intensity from the RRM. These pre-bound aptamer/nanoparticle conjugates were then exposed to BPA in free solution and the competitive binding event was monitored by the decrease in SERS intensity.

KW - aptamer

KW - competitive binding assay

KW - molecular diagnostics

KW - plasmonic nanoparticles

KW - Surface enhanced Raman spectroscopy (SERS)

UR - http://www.scopus.com/inward/record.url?scp=84931863348&partnerID=8YFLogxK

U2 - 10.1117/12.2078869

DO - 10.1117/12.2078869

M3 - Conference contribution book

SN - 9781628414288

T3 - Proceedings of SPIE

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

A2 - Parak, Wolfgang J.

A2 - Osinski, Marek

A2 - Liang, Xing-Jie

CY - San Francisco

ER -

Marks H, Mabbott S, Jackson GW, Graham D, Cote GL. SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. In Parak WJ, Osinski M, Liang X-J, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. San Francisco. 2015. 93381C. (Proceedings of SPIE). https://doi.org/10.1117/12.2078869