SERRS-based enzymatic probes for the detection of protease activity

A. Ingram, L. Byers, K. Faulds, B.D. Moore, D. Graham

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.
Original languageEnglish
Pages (from-to)11846-11847
Number of pages2
JournalJournal of the American Chemical Society
Issue number36
Publication statusPublished - 10 Sep 2008


  • SERRS-based
  • enzymatic probes
  • detection
  • protease activity

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