SERRS-based enzymatic probes for the detection of protease activity

A. Ingram, L. Byers, K. Faulds, B.D. Moore, D. Graham

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.
LanguageEnglish
Pages11846-11847
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number36
DOIs
Publication statusPublished - 10 Sep 2008

Fingerprint

Peptide Hydrolases
Azo Compounds
Subtilisins
Azo dyes
Phenylalanine
Limit of Detection
Substrates

Keywords

  • SERRS-based
  • enzymatic probes
  • detection
  • protease activity

Cite this

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SERRS-based enzymatic probes for the detection of protease activity. / Ingram, A.; Byers, L.; Faulds, K.; Moore, B.D.; Graham, D.

In: Journal of the American Chemical Society, Vol. 130, No. 36, 10.09.2008, p. 11846-11847.

Research output: Contribution to journalArticle

TY - JOUR

T1 - SERRS-based enzymatic probes for the detection of protease activity

AU - Ingram, A.

AU - Byers, L.

AU - Faulds, K.

AU - Moore, B.D.

AU - Graham, D.

PY - 2008/9/10

Y1 - 2008/9/10

N2 - Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.

AB - Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.

KW - SERRS-based

KW - enzymatic probes

KW - detection

KW - protease activity

U2 - 10.1021/ja803655h

DO - 10.1021/ja803655h

M3 - Article

VL - 130

SP - 11846

EP - 11847

JO - Journal of the American Chemical Society

T2 - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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