Serine proteases in rodent hippocampus

Ben J. Davies, Benjamin S. Pickard, Muriel Steel, Richard G. M. Morris, Richard Lathe

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Brain serine proteases are implicated in developmental processes, synaptic plasticity, and in disorders including Alzheimer's disease. The spectrum of the major enzymes expressed in brain has not been established previously. We now present a systematic study of the serine proteases expressed in adult rat and mouse hippocampus. Using a combination of techniques including polymerase chain reaction amplification and Northern blotting we show that tissue-type plasminogen activator (t-PA) is the major species represented. Unexpectedly, the next most abundant species were RNK-Met-1, a lymphocyte protease not reported previously in brain, and two new family members, BSP1 (brain serine protease 1) and BSP2. We report full-length sequences of the two new proteases; homologies indicate that these are of tryptic specificity. Although BSP2 is expressed in several brain regions, BSP1 expression is strikingly restricted to hippocampus. Other enzymes represented, but at lower levels, included elastase IV, proteinase 3, complement C2, chymotrypsin B, chymotrypsin-like protein, and Hageman factor. Although thrombin and urokinase-type plasminogen activator were not detected in the primary screen, low level expression was confirmed using specific polymerase chain reaction primers. In contrast, and despite robust expression of t-PA, the usual t-PA substrate plasminogen was not expressed at detectable levels.
LanguageEnglish
Pages23004-23011
Number of pages8
JournalJournal of Biological Chemistry
Volume273
DOIs
Publication statusPublished - 4 Sep 1998

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Serine Proteases
Rodentia
Hippocampus
Brain
Plasminogen Activators
Polymerase chain reaction
Peptide Hydrolases
Complement C2
Myeloblastin
Factor XII
Polymerase Chain Reaction
Neuronal Plasticity
Lymphocytes
Pancreatic Elastase
Plasminogen
Urokinase-Type Plasminogen Activator
Chymotrypsin
Tissue Plasminogen Activator
Enzymes
Thrombin

Keywords

  • amino acid sequence
  • animals
  • base sequence
  • brain chemistry
  • gene expression
  • hippocampus
  • nerve tissue proteins
  • molecular sequence data
  • rats

Cite this

Davies, B. J., Pickard, B. S., Steel, M., Morris, R. G. M., & Lathe, R. (1998). Serine proteases in rodent hippocampus. Journal of Biological Chemistry, 273, 23004-23011. https://doi.org/10.1074/jbc.273.36.23004
Davies, Ben J. ; Pickard, Benjamin S. ; Steel, Muriel ; Morris, Richard G. M. ; Lathe, Richard. / Serine proteases in rodent hippocampus. In: Journal of Biological Chemistry. 1998 ; Vol. 273. pp. 23004-23011.
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Davies, BJ, Pickard, BS, Steel, M, Morris, RGM & Lathe, R 1998, 'Serine proteases in rodent hippocampus' Journal of Biological Chemistry, vol. 273, pp. 23004-23011. https://doi.org/10.1074/jbc.273.36.23004

Serine proteases in rodent hippocampus. / Davies, Ben J.; Pickard, Benjamin S.; Steel, Muriel; Morris, Richard G. M.; Lathe, Richard.

In: Journal of Biological Chemistry, Vol. 273, 04.09.1998, p. 23004-23011.

Research output: Contribution to journalArticle

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