Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays

Ishwar Singh, Christian Wendeln, Alasdair W. Clark, Jonathan M. Cooper, Bart Jan Ravoo, Glenn A. Burley

Research output: Contribution to journalArticle

31 Citations (Scopus)
102 Downloads (Pure)

Abstract

We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch
Original languageEnglish
Pages (from-to)3449-3457
Number of pages9
JournalJournal of the American Chemical Society
Volume135
Issue number9
Early online date21 Feb 2013
DOIs
Publication statusPublished - 6 Mar 2013

Keywords

  • microarray format
  • double-stranded DNA sequences
  • sequence selectivity

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