Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer

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Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.
Original languageEnglish
Pages (from-to)2796-2798
Number of pages3
JournalApplied Physics Letters
Issue number18
Publication statusPublished - 30 Apr 2001


  • metabolites
  • donor-acceptor nano-distributions
  • fluorescence resonance
  • energy transfer
  • nanoscience
  • fluorescence resonance energy transfer
  • fret

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