Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.
LanguageEnglish
Pages2796-2798
Number of pages3
JournalApplied Physics Letters
Volume78
Issue number18
DOIs
Publication statusPublished - 30 Apr 2001

Fingerprint

metabolites
resonance fluorescence
energy transfer
glucose
distribution functions
fluorescence
kinetics

Keywords

  • metabolites
  • donor-acceptor nano-distributions
  • fluorescence resonance
  • energy transfer
  • nanoscience
  • fluorescence resonance energy transfer
  • fret

Cite this

@article{6a59706235f8425791c3f30b91096acf,
title = "Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer",
abstract = "Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.",
keywords = "metabolites, donor-acceptor nano-distributions, fluorescence resonance, energy transfer, nanoscience, fluorescence resonance energy transfer, fret",
author = "O.J. Rolinski and D.J.S. Birch and L.J. McCartney and J.C. Pickup",
year = "2001",
month = "4",
day = "30",
doi = "10.1063/1.1367308",
language = "English",
volume = "78",
pages = "2796--2798",
journal = "Applied Physics Letters",
issn = "0003-6951",
number = "18",

}

Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer. / Rolinski, O.J.; Birch, D.J.S.; McCartney, L.J.; Pickup, J.C.

In: Applied Physics Letters, Vol. 78, No. 18, 30.04.2001, p. 2796-2798.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer

AU - Rolinski, O.J.

AU - Birch, D.J.S.

AU - McCartney, L.J.

AU - Pickup, J.C.

PY - 2001/4/30

Y1 - 2001/4/30

N2 - Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.

AB - Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.

KW - metabolites

KW - donor-acceptor nano-distributions

KW - fluorescence resonance

KW - energy transfer

KW - nanoscience

KW - fluorescence resonance energy transfer

KW - fret

U2 - 10.1063/1.1367308

DO - 10.1063/1.1367308

M3 - Article

VL - 78

SP - 2796

EP - 2798

JO - Applied Physics Letters

T2 - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 18

ER -