Self-assembly of protein nanoarrays on block copolymer templates

K. H. A. Lau, J. Bang, D. H. Kim, W. Knoll

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

There is considerable interest in developing functional protein arrays on the nanoscale for high-throughput protein-based array technology, and for the study of biomolecular and cell interactions at the physical scale of the biomolecules. To these ends, self-assembly based techniques may be desirable for the nanopatterning of proteins on large sample areas without the use of lithography equipment. We present a fast, general approach for patterning proteins (and potentially other biomolecules) on the nanoscale, which takes advantage of the ability of block copolymers to self-assemble into ordered surface nanopatterns with defined chemical heterogeneity. We demonstrate nanoarrays of immunoglobulin and bovine serum albumin on polystyrene-block-poly(methyl methacrylate) templates, and illustrate the applicability of our technique through immunoassays and DNA sensing performed on the protein nanoarrays. Furthermore, we show that the pattern formation mechanism is a nanoscale effect originating from a combination of fluid flow forces and geometric restrictions templated by an underlying nanopattern with a difference in protein adsorption behavior on adjacent, chemically distinct surfaces. This understanding may provide a framework for extending the patterning approach to other proteins and material systems.
LanguageEnglish
Pages3148-3157
Number of pages10
JournalAdvanced Functional Materials
Volume18
Issue number20
DOIs
Publication statusPublished - 23 Oct 2008

Fingerprint

block copolymers
Self assembly
Block copolymers
self assembly
templates
proteins
Proteins
Biomolecules
immunoassay
Bovine Serum Albumin
Polymethyl methacrylates
albumins
polymethyl methacrylate
serums
Lithography
fluid flow
Immunoglobulins
Flow of fluids
Polystyrenes
constrictions

Keywords

  • proteins
  • self-assembly
  • arrays
  • block copolymers
  • nanopatterning

Cite this

Lau, K. H. A. ; Bang, J. ; Kim, D. H. ; Knoll, W. / Self-assembly of protein nanoarrays on block copolymer templates. In: Advanced Functional Materials. 2008 ; Vol. 18, No. 20. pp. 3148-3157.
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Self-assembly of protein nanoarrays on block copolymer templates. / Lau, K. H. A.; Bang, J.; Kim, D. H.; Knoll, W.

In: Advanced Functional Materials, Vol. 18, No. 20, 23.10.2008, p. 3148-3157.

Research output: Contribution to journalArticle

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