Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells

Mi Zhou, A.M. Smith, A.K. Das, N.W. Hodson, R.F. Collins, R.V. Ulijn, J.E. Gough

Research output: Contribution to journalArticle

426 Citations (Scopus)

Abstract

We report here the design of a biomimetic nanofibrous hydrogel as a 3D-scaffold for anchorage-dependent cells. The peptide-based bioactive hydrogel is formed through molecular self-assembly and the building blocks are a mixture of two aromatic short peptide derivatives: Fmoc-FF (Fluorenylmethoxycarbonyl-diphenylalanine) and Fmoc-RGD (arginine-glycine-aspartate) as the simplest self-assembling moieties reported so far for the construction of small-molecule-based bioactive hydrogels. This hydrogel provides a highly hydrated, stiff and nanofibrous hydrogel network that uniquely presents bioactive ligands at the fibre surface; therefore it mimics certain essential features of the extracellular matrix. The RGD sequence as part of the Fmoc-RGD building block plays a dual role of a structural component and a biological ligand. Spectroscopic and imaging analysis using CD, FTIR, fluorescence, TEM and AFM confirmed that FF and RGD peptide sequences self-assemble into beta-sheets interlocked by pi-pi stacking of the Fmoc groups. Ibis generates the cylindrical nanofibres interwoven within the hydrogel with the presence of RGDs in tunable densities on the fibre surfaces. This rapid gelling material was observed to promote adhesion of encapsulated dermal fibroblasts through specific RGD-integrin binding, with subsequent cell spreading and proliferation; therefore it may offer an economical model scaffold to 3D-culture other anchorage-dependent cells for in-vitro tissue regeneration.
LanguageEnglish
Pages2523-2530
Number of pages8
JournalBiomaterials
Volume30
Issue number13
DOIs
Publication statusPublished - May 2009

Fingerprint

Hydrogels
Hydrogel
Scaffolds (biology)
Scaffolds
Peptides
Ligands
Nanofibers
Tissue regeneration
Biomimetics
Fibers
Fourier Transform Infrared Spectroscopy
Fibroblasts
Arginine
Aspartic Acid
Integrins
Glycine
Self assembly
Extracellular Matrix
Regeneration
Adhesion

Keywords

  • minimalist design
  • self-assembly
  • biomimetic materials
  • rgd ligands
  • 3d culture
  • anchorage-dependent cells

Cite this

Zhou, M., Smith, A. M., Das, A. K., Hodson, N. W., Collins, R. F., Ulijn, R. V., & Gough, J. E. (2009). Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells. Biomaterials, 30(13), 2523-2530. https://doi.org/10.1016/j.biomaterials.2009.01.010
Zhou, Mi ; Smith, A.M. ; Das, A.K. ; Hodson, N.W. ; Collins, R.F. ; Ulijn, R.V. ; Gough, J.E. / Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells. In: Biomaterials. 2009 ; Vol. 30, No. 13. pp. 2523-2530.
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Zhou, M, Smith, AM, Das, AK, Hodson, NW, Collins, RF, Ulijn, RV & Gough, JE 2009, 'Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells' Biomaterials, vol. 30, no. 13, pp. 2523-2530. https://doi.org/10.1016/j.biomaterials.2009.01.010

Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells. / Zhou, Mi; Smith, A.M.; Das, A.K.; Hodson, N.W.; Collins, R.F.; Ulijn, R.V.; Gough, J.E.

In: Biomaterials, Vol. 30, No. 13, 05.2009, p. 2523-2530.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells

AU - Zhou, Mi

AU - Smith, A.M.

AU - Das, A.K.

AU - Hodson, N.W.

AU - Collins, R.F.

AU - Ulijn, R.V.

AU - Gough, J.E.

PY - 2009/5

Y1 - 2009/5

N2 - We report here the design of a biomimetic nanofibrous hydrogel as a 3D-scaffold for anchorage-dependent cells. The peptide-based bioactive hydrogel is formed through molecular self-assembly and the building blocks are a mixture of two aromatic short peptide derivatives: Fmoc-FF (Fluorenylmethoxycarbonyl-diphenylalanine) and Fmoc-RGD (arginine-glycine-aspartate) as the simplest self-assembling moieties reported so far for the construction of small-molecule-based bioactive hydrogels. This hydrogel provides a highly hydrated, stiff and nanofibrous hydrogel network that uniquely presents bioactive ligands at the fibre surface; therefore it mimics certain essential features of the extracellular matrix. The RGD sequence as part of the Fmoc-RGD building block plays a dual role of a structural component and a biological ligand. Spectroscopic and imaging analysis using CD, FTIR, fluorescence, TEM and AFM confirmed that FF and RGD peptide sequences self-assemble into beta-sheets interlocked by pi-pi stacking of the Fmoc groups. Ibis generates the cylindrical nanofibres interwoven within the hydrogel with the presence of RGDs in tunable densities on the fibre surfaces. This rapid gelling material was observed to promote adhesion of encapsulated dermal fibroblasts through specific RGD-integrin binding, with subsequent cell spreading and proliferation; therefore it may offer an economical model scaffold to 3D-culture other anchorage-dependent cells for in-vitro tissue regeneration.

AB - We report here the design of a biomimetic nanofibrous hydrogel as a 3D-scaffold for anchorage-dependent cells. The peptide-based bioactive hydrogel is formed through molecular self-assembly and the building blocks are a mixture of two aromatic short peptide derivatives: Fmoc-FF (Fluorenylmethoxycarbonyl-diphenylalanine) and Fmoc-RGD (arginine-glycine-aspartate) as the simplest self-assembling moieties reported so far for the construction of small-molecule-based bioactive hydrogels. This hydrogel provides a highly hydrated, stiff and nanofibrous hydrogel network that uniquely presents bioactive ligands at the fibre surface; therefore it mimics certain essential features of the extracellular matrix. The RGD sequence as part of the Fmoc-RGD building block plays a dual role of a structural component and a biological ligand. Spectroscopic and imaging analysis using CD, FTIR, fluorescence, TEM and AFM confirmed that FF and RGD peptide sequences self-assemble into beta-sheets interlocked by pi-pi stacking of the Fmoc groups. Ibis generates the cylindrical nanofibres interwoven within the hydrogel with the presence of RGDs in tunable densities on the fibre surfaces. This rapid gelling material was observed to promote adhesion of encapsulated dermal fibroblasts through specific RGD-integrin binding, with subsequent cell spreading and proliferation; therefore it may offer an economical model scaffold to 3D-culture other anchorage-dependent cells for in-vitro tissue regeneration.

KW - minimalist design

KW - self-assembly

KW - biomimetic materials

KW - rgd ligands

KW - 3d culture

KW - anchorage-dependent cells

U2 - 10.1016/j.biomaterials.2009.01.010

DO - 10.1016/j.biomaterials.2009.01.010

M3 - Article

VL - 30

SP - 2523

EP - 2530

JO - Biomaterials

T2 - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 13

ER -